by immunizing mice with killed whole bacterial cells of brucella abortus s (99), a panel of six hybridomas producing monoclonal antibodies (mab) specific for the surface antigens of this bacterium were produced. elisa was used to screen the hybridoma supernatants. immunoblots of the cell extract indicated that three mab were specific for s-lps (ba-1, ba-2, ba-3) and three others were reactive w...

the first description of an autoimmune encephalitis dates back to 1888, when hermann oppenheim described a patient with neurological symptoms but no underlying brain pathology. the field of autoimmune encephalitides associated with antibodies targeting cell-surface antigens is rapidly expanding and new antibodies are discovered frequently. autoimmune encephalitis is a difficult clinical diagnos...

r-phycoerythrin (r-pe), a fluorescent protein from phycobiliprotein family, is isolated from red algae. conjugation of antibodies to r-pe facilitates multiple fluorescent staining methods. in the present study polyclonal antibodies and polyclonal f(ab')2 fragment antibodies were conjugated to r-pe by two different methods. the efficiency of the methods was evaluated using immunocytochemistry (i...

to study the humoral immune response to entamoeba histolytica infection, sera prepared from different cases of amoebiasis from gastroenterology ward of b.h.u hospital, including15 cases with amoebic liver abscess (ala), 10 cases with acute amoebic dysentery (ad) and 10 controls were examined by enzyme linked immuno sorbent assay (elisa), using crude amoebic antigen prepared from entamoeba histo...

background : production of monoclonal antibodies to leishmania antigens assists the identification and characterization of these organisms. objective: production of monoclonal antibodies against epitopes on the gp63. methods: two murine monoclonal antibodies to gp63 were produced and characterized. the reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

background: evidences suggest an association between the prevalence of thyroid peroxidase (anti-tpo) and anti thyroglobulin (anti-tg) with recurrent abortions and infertility. iodine deficiency was once endemic in iran and little data is available about the prevalence of these antibodies in different groups of fertile or infertile individuals. objective: this case control study was designed to ...

The phofoinduccd rransjcis isornerization is one of the 111051 important fundamental processes in photoclwlttical and photobiological reactions. and many invcsrigarions have been carried out in order to elucidate its mechanism. Recently. direct observation of rhe isomcrization process in rhe singlet excited state 113s become possible owing to the development of p~i~~~md iascr spectroscopy. Up 1...

The knowledge of the microscopic structure of water at interfaces is essential for the understanding of interfacial phenomena in numerous natural and technological environments. To study deeply buried liquid water-solid interfaces, high-energy x-ray reflectivity measurements have been performed. Silicon wafers, functionalized by a self-assembled monolayer of octadecyl-trichlorosilane, provide s...

background blood transfusion is one of the many ways, through which toxoplasma gondii (t. gondii), a protozoan parasite, can be transmitted to humans. objectives this cross sectional study aimed to evaluate the seroprevalence of toxoplasma infection and related epidemiological features among healthy blood donors. materials and methods a total of 375 blood samples from donors were tested for spe...