Background: Ovarian carcinoma is one of the leading causes of cancer-related death among females. K-ras codon 12 mutations are commonly occurring mutations in different types of cancers and leads to resistance against anti-EGFR therapeutics. Hence, determination of mutations in k-ras gene is crucial for predicting response to anti-EGFR therapies. This study aimed to evaluate the prevalence of k...

The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and specificity; thus, many small medium laboratories use it to perform all kinds single nucleotide polymorphisms (SNPs) genotyping works, as a molecular biotechnology for disease-related analysis. However, lack ava...

Background: RhD antigen system is the leading cause of hemolytic disease of the fetus and newborn (HDFN). Paternal molecular RhD zygosity test is valuable to decide on the use of anti-D immunoglobulin prophylaxis in Rh D-negative pregnant women. We aimed to investigate the paternal RhD zygosity by two molecular methods among blood donors in Kurdistan province, the west of Iran. We also compared...

background: the worldwide distribution of p. vivax has expanded significantly and the number of reported cases has been on the rise. approximately 88% of malaria cases in iran are caused by plasmodium vivax , and in order to management of the disease, understanding the population genetic structure of the parasite is necessary for designing and applying drugs and vaccines. among many potential c...

background: toxoplasmosis is one of the most important food-borne diseases distributed worldwide. in order to determinate the involvement of poultry meat in clinical toxoplasmosis, this study aimed to genetic characterization of toxoplasma gondii in tissue samples of serologically positive chickens of tunisia using polymerase chain reaction (pcr). methods: trail of isolation of local t. gondii ...

CAST gene is a candidate marker that influences the intensity of growth and meat quality. The aim this study was to investigate genetic variability in five Bulgarian sheep breeds – two merino, local one for milk. A total 150 ewes, belonging these were investigated polymorphisms by using PCR-RFLP method. 622 bp fragment Ovis aries amplified PCR. After restriction with endonuclease MspI alleles o...

Background: The phosphatidylinositol 3-kinases are known as a family of lipid kinases, playing role in various cellular processes. A member this is PIK3CA which frequently mutated breast cancer. In study, the association between H1047R, E542K and E545K mutations, therapy resistance was investigated Iranian cancer patients treated by Trastuzumab. Methods: Resistance sensitive groups were chosen ...

Breast cancer (BC) is the most frequent in women globally and main cause of cancer-related mortality. MTHFR C677T a functional polymorphism genes encoding one-carbon metabolism enzyme that affect folate may be associated with breast susceptibility. We have conducted case-control study taking 100 cases diagnosed healthy control. PCR-RFLP technique was used to detect genotype population. About 35...

Identification of polymorphism is the key for genetic mapping, diagnosis and marker-assisted selection. Restriction fragment-length polymorphisms (RFLPs) and short tandem repeats (STRs), also known as microsatellites, are commonly used genetic markers. Until discovery of STRs, RFLPs were used for genetic mapping, but bi-allelic polymorphism of RFLP limited its use as a marker. Although STRs are...