GPR103 is a G-protein-coupled receptor with reported expression in brain, heart, kidney, adrenal gland, retina, and testis. It encodes a 455-amino-acid protein homologous to neuropeptide FF2, neuropeptide Y2, and galanin GalR1 receptors. Its natural ligand was recently identified as 26RFa, a novel human RF-amide-related peptide with orexigenic activity. To identify the function of GPR103, we ge...

The field of peptide derivatization by metal-catalyzed C−H activation has been mostly directed to modify the side chains, but poor attention given backbone. Here we report a ruthenium-catalyzed activation/annulation process that can chemoselectively backbone producing functionalized isoquinolone scaffolds with high regioselectivity in rapid and step-economical manner. This strategy is character...

The chiral specificity of the interactions of peptides that induce the formation of cholesterol-rich domains has not been extensively investigated. Both the peptide and most lipids are chiral, so there is a possibility that interactions between peptide and lipid could require chiral recognition. On the other hand, in our models with small peptides, the extent of folding of the peptide to form a...

The α-helix – a common building block of the protein secondary structure – plays an important role in determining protein structure and function. The biological function of the α-helix is mainly attributed to its large macrodipole originating from the alignment of individual dipole moments of peptide bonds. Preparation of directionally aligned α-helical peptide layers on substrates has attracte...

To further improve fertility of animals, a novel gene RFRP-3 (RF-amide related peptide-3, RFRP-3) was used to construct DNA vaccines with INH α (1-32) (inhibin, INH) fragment for the first time. The aim of this study was to evaluate the effects of novel DNA vaccines on fertility in mice. Synthesized SINH and SRFRP (INH and RFRP genes were separately ligated to the C-terminus of the small envelo...

The rotavirus nonstructural protein 4 (NSP4), is a multi functional protein that play key role in both viral morphogenesis and cytopathic effect associated with cell death. However, the complete biological effect of NSP4 remains to be clarified. Since to obtain further knowledge about this protein there is a need for recognizing antibody and there is no commercial antibody against this protein,...

Our goal was to explore the possible interactions of potential metallodrug (η5-Cp*)Rh(III) complexes with histidine containing biomolecules (peptides/proteins) in order understand most important thermodynamic factors influencing biospeciation and biotransformation complexes. To this end, here we report systematic solution structural study on interaction cation peptides their constituents ((N-me...

Combined potentiometric, calorimetric and spectroscopic methods were used to investigate the Cu(2+) binding ability and coordination behaviour of some peptide fragments related to the neurotoxic region of chicken Prion Protein. The systems studied were the following protein fragments: chPrP(106-114), chPrP(119-126), chPrP(108-127), chPrP(105-127) and chPrP(105-133).The complex formation always ...

Introduction Amide proton transfer (APT) imaging is an application of chemical exchange saturation transfer (CEST) imaging that generates contrast from exchangeable protons on the backbone of endogeneous mobile proteins and peptides. In a conventional APT experiment, a label image is acquired with RF irradiation of amide protons at 3.5ppm, and subtracted from a reference image with RF irradiati...

the rotavirus nonstructural protein 4 (nsp4), is a multi functional protein that play key role in both viral morphogenesis and cytopathic effect associated with cell death. however, the complete biological effect of nsp4 remains to be clarified. since to obtain further knowledge about this protein there is a need for recognizing antibody and there is no commercial antibody against this protein...