نتایج جستجو برای: reverse transcriptase polymerase chain reaction

تعداد نتایج: 757054  

Journal: :Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 2015
M Rondy O Launay J Puig-Barberà G Gefenaite J Castilla K de Gaetano Donati F Galtier E Hak M Guevara S Costanzo A Moren

While influenza vaccines aim to decrease the incidence of severe influenza among high-risk groups, evidence of influenza vaccine effectiveness (IVE) among the influenza vaccine target population is sparse. We conducted a multicentre test-negative case-control study to estimate IVE against hospitalised laboratory-confirmed influenza in the target population in 18 hospitals in France, Italy, Lith...

Journal: :Genetics and molecular research : GMR 2015
L M Mu W F Wang H Zheng Z K Guo G M Zhang

We investigated the expression and distribution of N-cadherin during the development of a rat heart. Immunohistochemistry (IHC) was performed to detect the expression and distribution of N-cadherin in the myocardial tissues of rats at embryonic day 18 (E18d), postnatal day 5 (P5d), postnatal day 19 (P19d), postnatal day 40 (P40d), and postnatal year 1 (P1y). Reverse transcription polymerase cha...

2014
Young-Jin Park Dae-Eun Yoon Hong-Il Kim O-Chul Kwon Young-Bok Yoo Won-Sik Kong Chang-Soo Lee

Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among various conditions, the best concentration of apple pomace and cultivation time for the production of laccase by P. ostreatus was 2.5% and 9 days, respectively. Reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1, pox3, pox4, poxc, poxa3, and ...

2014
Deepti Parashar Mandar S. Paingankar A. B. Sudeep Ashwini More Sapana B. Shinde Vidya A. Arankalle

The definitive diagnosis ability of IgM ELISA, nested RT-PCR and real-time quantitative PCR (qPCR) was evaluated for Chikungunya diagnosis using 180 clinical samples. Real-time qPCR showed a higher sensitivity (88.3%) for Chikungunya diagnosis in the early stages of infection, while IgM ELISA proved sensitive for the late stages of illness (81.8%). The results suggest that the application of bo...

Journal: :Bio Systems 2000
S Fuhrman M J Cunningham X Wen G Zweiger J J Seilhamer R Somogyi

A major challenge in the field of functional genomics is the development of computational techniques for organizing and interpreting large amounts of gene expression data. These methods will be critical for the discovery of new therapeutic drug targets. Here, we present a simple method for determining the most likely drug target candidates from temporal gene expression patterns assayed with rev...

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