macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

background : st6gal1, cox-2 and hb-egf genes are involved in different tumors and their enhanced expressions often correlate with poor prognosis. in this study we assay the expressions of these genes by reverse transcriptase-pcr in 54 breast cancer samples. methods : tissue samples were either formalin-fixed for histopathological examination or frozen for reverse transcriptase-pcr. image progra...

Background: Hyperuricemia contributes to kidney injury, characterized by tubular injury with epithelial–mesenchymal transition (EMT). Wnt5a/Ror2 signaling drives EMT in many kidney pathologies. This study sought to evaluate the involvement of Wnt5a/Ror2 in hyperuricemia-induced EMT in kidney tubular injury.Methods: A hyperuricemia model was performed in male Swiss background mice (3 months old,...

This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScript reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme has demonstrated higher thermal stability and is described as suitable to make long cDNA with a compl...

Objective: To establish the accuracy of computed tomography(CT) chest in diagnosis COVID-19 pneumonia by taking reverse transcriptase-polymerase chain reaction(RT-PCR) as a reference standard and to analyze discordant CT RT-PCR results.Materials Methods: A retrospective cross-sectional study patients presented tertiary health care hospital Punjab, Pakistan for examination with suspicion from Ap...

The present study was carried out to detect the circulating Newcastle disease virus (NDV) from various field outbreaks in Assam during 2018-2021. Nine hundred ninety-two clinical and post mortem samples were collected backyard commercial poultry subjected molecular detection by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). NDV could be detected 445 (n=757, 58.78%) tissue 53 (n=235, ...

List of non-standard abbreviations ATSDR Agency for Toxic Substances and Disease Registry GSK-3β Glycogen synthase kinase-3β Kim-1 Kidney injury molecule-1 RT-PCR Reverse transcriptase polymerase chain reaction

We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.

ARI: Acute Respiratory Infections; URTIs: Upper Respiratory Tract Infections; IVs: Influenza Viruses; RSV: Respiratory Syncytial Virus; PIVs: Parainfluenza Viruses; KIPyV: KI Polyomaviruses; WUPyV: WU Polyomaviruses; hMPV: human Metapneumovirus; KEMRI: Kenya Medical Research Institute; GC: Gonococci ; WHO: World Health Organization; CDC; Centers for Disease Control; cDNA: complimentary DNA; RT-...

Quality management and influential factors for the detection of single metastatic cancer cells by reverse transcriptase polymerase chain reaction [Review]. Eur J Clin Chem Clin Biochem 1997;35:3–10. 14. Bostick PJ, Chatterjee S, Chi DD, Huynh KT, Giuliano AE, Cote R, Hoon DS. Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the ly...