Telomere synthesis in most organisms depends on the action of the telomerase enzyme, which contains an RNA subunit that is stably associated with the reverse transcriptase subunit as well as additional telomerase proteins. In the budding yeast Saccharomyces cerevisiae, several structural domains that are responsible for mediating protein interactions with the telomerase RNA TLC1 have been ident...

Heparin inhibited the activities of: (a) reverse transcriptase from simian sarcoma virus; (b) DMA polymerases a, (i, and y from phytohemagglutinin-stimulated normal hu man lymphocytes, human leukemic lymphoblasts, and Molt-4 cells which are of acute lymphoblastic leukemia origin; and (c) terminal deoxynucleotidyl transferase from the two latter cell types. A 10-fold excess of enzyme overcame he...

Three mutants of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (V106A, V179D, and Y181C), which occur in clinical isolates and confer resistance to nonnucleoside reverse transcriptase inhibitors (NNRTIs), were analyzed for RNA- and DNA-dependent DNA polymerization and RNase H cleavage. All mutants demonstrated processivities of polymerization that were indistinguishable from...

Heterologous expression of human LINE-1 ORF2 in yeast yielded a single polypeptide (Mr145 000) which reacted with specific antibodies and co-purified with a reverse transcriptase activity not present in the host cells. Various deletion derivatives of the ORF2 polypeptide were also synthesized. Reverse transcriptase assays using synthetic polynucleotides as template and primer revealed that ORF2...

BACKGROUND Non-nucleoside reverse transcriptase inhibitors (NNRTIs) are one of the key components in highly active anti-retroviral therapy because of their high specificity and less toxicity. NNRTIs inhibit reverse transcriptase enzyme by binding to the allosteric site, which is 10Å away from the active site. Rapid emergence of resistance is the major problem with all anti-HIV agents. Hence, th...

AIDS (acquired immunodeficiency syndrome) is a potentially life-threatening infectious disease caused by human virus (HIV). To date, thousands of people have lost their lives annually due to HIV infection, and it continues be big public health issue globally. Since the discovery first drug, Zidovudine (AZT), nucleoside reverse transcriptase inhibitor (NRTI), 30 drugs been approved FDA, primaril...

Nucleoside reverse transcriptase inhibitors suppress HIV replication by blocking reverse transcriptase, an RNA-dependent DNA polymerase. These drugs can also affect cellular and mitochondrial DNA polymerases. Mitochondrial DNA polymerase gamma is particularly sensitive to nucleoside reverse transcriptase inhibitors, and the majority of adverse effects caused by nucleoside reverse transcriptase ...