RAF1 (c-raf) has been known as an important tumor-promoter in many cancers. However, the regulatory mechanisms affecting expressions are rarely reported. This study aimed to predict candidate miRNAs of gene and verify their functions progression lung cancer. The expression miR-643 was detected by reverse transcription polymerase chain reaction (RT-PCR). A dual-luciferase report system used rela...

Abstract A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect passion fruit, citrus-associated rhabdovirus (CiaRV), East Asian Passiflora virus (EAPV), latent (PLV), Telosma mosaic (TeMV). The optimized parameters included primer concentration, annealing temperature...

To describe the characteristics of neonates born to mothers with confirmed Covid-19 infection. A descriptive retrospective study using medical record data patients treated in neonatal intensive care unit (NICU) Dr. Soetomo Regional General Hospital from January 1 July 24, 2021. total 174 were positive COVID-19 reverse transcription-polymerase chain reaction (RT-PCR) swab test results. 11 RT-PCR...

The hologic panther fusion (PF) platform provides fully automated CE marked diagnostics for respiratory viruses, including the recently discovered severe acute syndrome-coronavirus 2 (SARS-CoV-2) by a transcription mediated amplification (TMA) assay, but not endemic human coronaviruses (hCoV). Therefore, laboratory developed test (LDT) comprising multiplexed reverse polymerase chain reaction (R...

The article describes a polymerase chain reaction (PCR) based method for semiquantitative assessment of mRNA expression for chemotactic cytokines in the lower respiratory tract cells. Cytokine mRNA was detected using PCR preceded by reverse transcription of mRNA into cDNA. The procedures of RNA extraction and of reverse transcription-PCR assay are described in the first part of the article. The...

Reoviruses have been isolated from many aquatic animals including fish and crustaceans. Viral inclusion bodies of reovirus found in the cytoplasm hepatopancreatic cells redclaw crayfish (Cherax quadricarinatus). In past, reverse transcription polymerase chain reaction (RT-PCR) designed across other hosts was attempted to detect but no specific set primers successfully identified infected crayfi...

Abbreviations: ALT: Alanine Transaminase; AST: Aspartate Transaminase; BMI: Body Mass Index; CMV: Cytomegalovirus; Cnis: Calcineurin Inhibitors; Csa: Cyclosporine; FK Or FK-506: Tacrolimus; GRWR: Graft Recipient Weight Ratio; HAI: Histological Activity Index; HCC: Hepatocellular Carcinoma; HCV: Hepatitis C Virus; HCV RNA: Hepatitis C Virus Ribonuclic Acid; HPB: Hepatopancreatobiliary;LDLT: Livi...