A method for quantitative determination of tetracycline in serum, using high-pressure liquid chromatography, is reported. After extraction of the drug from serum, using methanol-trichloroacetic acid solution, tetracycline was separated by reverse-phase chromatography. Quantitation of tetracycline was based on a linear relationship between peak heights in the chromatograms and known concentratio...

aflatoxins (afs) are one of the most important mycotoxins due to their common occurrence in feedstuffs and feeds that pose a serious threat to humans and animals. although many outbreaks of acute and chronic diseases have been attributed to consumption of aflatoxin-contaminated foods, the most significant effect of aflatoxin is hepatotoxicity in farm animals, especially aflatoxicosis in shrimps...

A sensitive, accurate, precise and validated ion-pairing reverse-phase liquid chromatographic method for the quantitative determination of atenolol and indapamide in bulk and tablet dosage form was developed. The proposed ion-pairing reverse-phase high performance liquid chromatography method utilises C(18) column with 5 μm, 150×4.6 mm i.d. column and mobile phase consisting of 0.1% w/v solutio...

Our objective was to establish a system for the investigation of neuropeptide biosynthesis in an insect. To achieve this we developed an in vitro organ culture system for the corpora cardiaca (CC) of the locust (Schistocerca gregaria). The CC are the neurosecretory structures containing the adipokinetic hormones AKH I and AKH II. Tritiated amino acids were added to the glands, and we studied th...

A new technique for separation and dctermination of ATP, ADP, AMP, NAD', NADP', NADH and NADPH in Lactobacillus plantarum has been developped. It involves acid and basic extraction of nucleotide pool, analysis by reverse-phase high performance liquid chromatography on a 5 p Spherisorb ODS-1 column and UV detection. The method offers advantages in accurate estimations of adenylic and nicotinamid...

Riboflavln (Vitamin 82) in fresh fruits and vegetables, which is present in ng levels, was separated on a reverse-phase Altex-ultrasphere ODS column with water, methanol, and heptane sulfonic acid as the mobile phase. Ion-pairing reduced tailing of the peak. Quantity of riboflavin was based on fluorescence, excitation at 450 nm, and emission at 530 nm. INTRODUCTION Analysis of riboflavin has be...

Avilamycin is an antibiotic complex produced by cultures of the organism Streptomyces viridochromogenes, strain NRRL 2860. These compounds belong to the orthosomycin family of antibiotics. Structural composition of the major avilamycins and several minor avilamycins are known. Nine additional minor avilamycins, designated F through N, have been isolated via semi-preparative silica gel or revers...

Reverse micellar systems are nanofluids with unique properties that make them attractive in high selectivity separation processes, especially for biological compounds. Understanding the phase behavior and thermodynamic properties of these nanosystems is the first step in process design. Separation of components by these nanosystems is performed upon contact of aqueous and reverse micellar phase...

[3H]Myristoyl-labeled variant surface glycoprotein (VSG) has been isolated from Trypanosoma brucei by reverse phase high performance liquid chromatography and used as substrate for the conversion by trypanosomal enzymes of membrane-form VSG to soluble VSG. Conversion is detected by the release of myristoyl-containing lipids. The major lipolytic enzyme of T. brucei, phospholipase A1, is effectiv...

A improved, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated as per ICH guidelines for the quantification of acetic acid present in Gefitinib as an solvent impurity. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible. The high recovery and low relative st...