BACKGROUND Imaging reporter gene expression is useful for noninvasive monitoring of gene therapy. In this study, we imaged cardiac reporter gene expression in living rats using micro positron emission tomography (microPET). METHODS AND RESULTS Rats (n=10) underwent intramyocardial injection with 1x10(9) pfu of adenovirus carrying cytomegalovirus promoter-driving herpes simplex virus type 1 mu...

Introduction: The ecdysone inducible system potentially allows the study of conditional expression of the exogenous reporter genes that may be cell lethal or alter the phenotype during the selection of transfectants. The system relies on two independent transfections of plasmids named pVgRXR and pIND. Disruption of the regulatory element within the plasmid during stable integration can result i...

The lack of toxicological information on many of the compounds that humans use or are exposed to, intentionally or unintentionally, poses a big problem in risk assessment. To fill this data gap, more emphasis is given to fast in vitro screening tools that can add toxicologically relevant information regarding the mode(s) of action via which compounds can elicit adverse effects, including genoto...

Histone chaperone is important for nucleosome assembly during DNA replication. Interestingly, recent efforts have shed light on the role of histone chaperones in aggressive progression multiple cancers. Though ASF1B plays a critical replication and cancer progression. Nevertheless, underlying mechanism regulatory network gastric remain largely unknown. Bioinformatics approach was used to identi...

mucopolysaccharidoses are a group of rare mostly autosomal recessive metabolic and genetic disorders. because of the high rate of consanguinity, they are not uncommon in our population. clinical diagnosis is not difficult for experienced physicians, but it is not enough for treatment and prevention measures in the future pregnancies. so, it is necessary to detect the type of mps by measurement ...

Previously, we have reported the use of a recombinant adenovirus (Ad)-based host cell reactivation (HCR) assay to examine nucleotide excision repair (NER) of UVC-induced DNA lesions in several mammalian cell types. The recombinant non-replicating Ad expresses the Escherichia coli β-galactosidase (β-gal) reporter gene under control of the cytomegalovirus immediate-early enhancer region. We have ...

OBJECTIVE To examine the effects of thrombin on RANTES mRNA expression through protease activated receptor in synovial fibroblasts in patients with rheumatoid arthritis (RA). METHODS A semiquantitative reverse transcriptase-polymerase chain reaction and reporter gene assay were performed using cultured human synovial fibroblasts from patients with RA. The up regulatory effects of thrombin on ...

In the postgenomic era, imaging techniques are playing an important role in visualizing gene expression in vivo. This work represents the first demonstration of photoacoustic tomography (PAT) for reporter gene imaging. Rats inoculated with 9L/lacZ gliosarcoma tumor cells are imaged with PAT before and after injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme beta-galactosidase....