Mitochondrial biogenesis is a cell response to external stimuli which generally believed suppress apoptosis. However, during the process of apoptosis, whether mitochondrial occurs in early stage apoptotic cells remains unclear. To address this question, we constructed COX8-EGFP-ACTIN-mCherry HeLa with recombinant fluorescent proteins respectively tagged on nucleus and mitochondria, monitored ch...

Background Toxic effects of anti-cancer and other drugs on the normal tissues could be reduced by the herbal plants and their fractions. This study investigated the protective effect of thymoquinone (TQ) as a fraction of Nigella sativa on methotrexate (MTX)- induced germ cell apoptosis in male mice. MaterialsAndMethods In this experimental study, thirty male Balb/c mice were divided randomly in...

objective(s): activation of acid-sensing ion channel 1a (asic1a) is responsible for tissue injury caused by acidosis in nervous systems. but its physiological and pathological roles in nucleus pulposus cells (npcs) are unclear. the aim of this study is to investigate whether asic1a regulates the survival of npcs in the acidic environment of degenerated discs. materials and methods: npcs were is...

objective: the anti-cancer properties of curcumin, a poliphenol extract from the rhizome of curry, has been confirmed by many investigators. however, low levels of uptake, tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. this study is aimed at increasing curcumin's water solubility due to a biodegradable, neutral and non-toxic micellar nano-carrier ca...

The purpose of this study was to evaluate the effect preventive acupuncture combined with CMNS6-1 electroacupuncture on 7,12-dimethylbenzanthracene induced mammary tumors in Sprague-Dawley rats and explore from perspective apoptosis. 48 w old female were randomly divided into control (10), model tamoxifen (10) groups. group, group administered once intragastrically 100 mg/kg body weight induce ...

Emerging evidence that Enterovirus A 71 (EV-A71) infection closely related to apoptosis. The ZVAD is a caspase inhibitor can prevent aims of this project were evaluate the mechanism inhibited EV-A71 virus and provide experiment basis for finding new antiviral drugs. In study, after treated with in infected Vero cells, viral replication was reduced, cell viability higher than group. Additionally...

To explore the effect and mechanism of Danggui Buxue decoction on high glucose-induced apoptotic oxidative injury in human retinal vascular endothelial cells. Human cells were treated with glucose to mimic diabetic retinopathy condition vitro. The 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl-2H-tetrazolium bromide assay flow cytometry used investigate cell viability apoptosis. Western blot was ap...

Bcl-2 family proteins are key intracellular regulators of programmed cell death. Several recent discoveries demonstrate how these proteins interact with the molecular machinery that controls and executes the cell-death programme, and how they can themselves be regulated by extracellular survival signals.