The electrochemical behaviour of SWCNTs randomly dispersed on gold and glassy carbon electrode surfaces was characterised via cyclic voltammetry and complex impedance spectroscopy, using the ferri/ferrocyanide couple as a redox active test probe . In subsequent investigations glucose oxidase (GOx) was adsorbed onto the SWCNT ensemble without apparent denaturation of the enzyme. Cyclic voltammet...

Superoxide Dismutase-Based Third-Generation Biosensor for Superoxide Anion Electrochemical Immunoassay Moving into the Fast Lane Characterization and Reactions of Previously Elusive 17Electron Cations: Electrochemical Oxidations of (C H )Cr(CO) and (C H )Co(CO) in the Presence of [B(C F ) ] Although chemical redox reagents can be highly selective, many produce toxic b y p r o d u c t s . I n c ...

The direct electrochemistry of human, bovine and porcine cytochrome P450c17 (CYP17) has been examined on an edge-oriented pyrolytic graphite electrode. The recombinant protein was immobilized on an electrode modified with a surfactant to simulate the environment of a biological membrane, and hence physiological electron-transfer conditions. The P450 enzymes all retained 'electron-transfer' acti...

A portable vacuum interface allowing direct probing of the electrode-electrolyte interface was developed. A classical electrochemical system consisting of a gold working electrode, platinum counter electrode, platinum reference electrode, and potassium iodide electrolyte was used to demonstrate real-time observation of the gold iodide adlayer on the electrode and chemical species as a result of...

In this study, we developed a micropyramid array electrode that facilitates contact between a nitrocellulose membrane and electrode, which is important to realize a quantitative and sensitive electrochemical detection system for immunochromatography. We evaluated the micropyamid array electrode with our newly developed detection system that can measure contact forces between a membrane and elec...

The aim of this work was to demonstrate that interaction of ferric cations with poly(o-aminophenol) (POAP) films causes their deactivation. Spectroelectrochemical measurements and rotating disk electrode voltammetry (RDEV) were employed on both freshly prepared and deactivated POAP films. It was observed a reduction (deactivation) of the redox activity of POAP after its treatment with ferric ca...

This article reviews recent progress in the development of ferrocene (Fc)-modified thin films and nanoparticles in relation to their biosensor applications. Redox-active materials in enzyme biosensors commonly use Fc derivatives, which mediate electron transfer between the electrode and enzyme active site. Either voltammetric or amperometric signals originating from redox reactions of Fc are de...

Scannlng electrochemical microscopy (SECM) in the feedback mode, where the steady-state faradaic current at a mlcrodlsk electrode tip Is measured as the tip Is scanned close to a surface, was used to lnvestlgate several dlfferent polymer films on electrode surfaces: poly( vlnylterrocene), N,N’-Ms[3-(t))prop~lE4,4‘-blp~ridkdun e r n e , and Naflon containing Os(bpy);+. The tip response (Le., pos...

Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer conce...