The superoxide (O2-)-forming NADPH oxidase of resting macrophages can be activated in a cell-free system by certain anionic amphiphiles, such as sodium dodecyl sulfate (SDS). O2- production requires the cooperation of membrane-associated and cytosolic components. The membrane component can be solubilized by octyl glucoside yielding a highly active oxidase preparation. High performance gel filtr...

1. Zhou J, Ditangco R. Predict immune reconstitution syndrome [letter]. Clin Infect Dis 2006; 43:147–8 (in this issue). 2. Robertson J, Meier M, Wall J, et al. Immune reconstitution syndrome in HIV: validating a case definition and identifying clinical predictors in persons initiating antiretroviral therapy. Clin Infect Dis 2006; 42:1639–46. 3. Zhou J, Kumarasamy N, Ditangco R, et al. The TREAT...

The fabrication of a thermoresponsive biohybrid double hydrophilic block copolymer (DHBC) by a cofactor reconstitution approach is reported. Poly(N-isopropylacrylamide) (PNIPAM) bearing a porphyrin moiety at the chain terminal, PPIXZn-PNIPAM, is synthesized by the combination of ATRP and a click reaction. The subsequent cofactor reconstitution process between apomyoglobin and PPIXZn-PNIPAM affo...