Stevia rebaudiana (Bert.) produces steviol glycoside sweeteners that are sweeter than sucrose. Stevioside and rebaudioside A important glycosides used in food production. This study was conducted to investigate the effect of different concentrations cadmium chloride (0, 20, 40, 60 80 mg/L) silver nitrate 15, 30, 45 sampling times on stevioside production Ent-kaurenoic acid 13-hydroxylase, uridi...

A plant tissue culture protocol from stevia was optimized for the production of planting materials and natural sweetener, rebaudioside A. The highest survivability (88.90% ± 5.55) explants achieved at 15 30 days after initiation (DACI) on Murashige Skoog (MS) media by sterilization with 30% Clorox (5 min) 10% (10 min), respectively. Supplementation MS 0.50 mg/L 2,4-Dichlorophenoxyacetic acid (2...

Abstract In this study, the optimization and performance evaluation of a high-performance liquid chromatography with diode array detection (HPLC–DAD) method for simultaneous determination four sweeteners (acesulfame-potassium, saccharin, aspartame, rebaudioside A), two preservatives (sodium benzoate potassium sorbate), caffeine in sugar-free drinks are presented. The separation was carried out ...

Hybridization is an important method to widen variations and develop novel varieties in plants. The increasing interest Stevia rebaudiana over the last decade soared by its potential as alternative source of sugar. In this study, improvement stevia has been conducted through hybridization using Trigona for development hybrids. Trigona, which consists small medium-sized bees, usually found tropi...