Molecular characterization of locally isolated Beauveria bassiana (Cordycipitaceae, Hypocreales) strains and their pathogenicity against Ceratitis capitata (Diptera: Tephritidae) adults are herein reported for the first time. Molecular identification based on the phylogenetic analyses of ITS-rDNA and combined data of TEF1 and Bloc, revealed that all fungal strains were conclusively assigned to ...

The RAPD method (Random Amplified Polymorphic DNA) was used for identifying and mapping new molecular markers in pea. RAPD analysis of various cultivars and lines of pea was carried out using 10-mer random primers. The presence of multiple polymorphism between cultivars and lines was revealed; at least one fragment for any given primer was present in the DNA of one form of pea and absent in the...

The genetic diversity of 20 Iranian populations of Bunium persicum has been evaluated with random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers. Fresh leaves of seedlings from each population were used for genomic DNA extraction. Analysis of banding patterns of 15 RAPD primers and 17 AFLP primer combinations, revealed 192 (86%) and 228 (75%) polymorp...

OBJECTIVES To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. METHODS Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in S...

The field experiments of the plant materials used in this investigation was carried out in Egypt during (2002-2005) while, 21 cotton genotypes were subjected to RAPD analysis using 53 UBC 10-mer RAPD primers in (2006) in Lab of Cotton Genetics and Breeding (Department of Agronomy and Horticulture) New Mexico State University, USA. Twenty three primers showed good amplification of cotton genome ...

Successful conservation of fish such as Plectropomus species depend on analysis of genetic signature and relations among fish species and subspecies. Knowledge about the genetic signature and structure of Plectropomus species needs to increase several times to enable us for conserve these economic fish in the future. The current study aims to develop and analysis of genetic signature for three ...

DNA based RAPD (Randomly Amplification of Polymorphic DNA) markers have been used extensively to study genetic relationships in number of crop plants. In this study, 44 okra genotypes collected from different parts of India, were selected to assess genetic distinctiveness and relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 14 arbitrary 10 mer primers. The molec...

Jatropha curcas L. is a rapidly emerging biofuel crop attracting a lot of interest, triggering large investments and rapid expansion of cultivation areas. In the present investigation, the genetic relationships of 29 J. curcas accessions were assessed based on randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses. A total of 72 polymorphic primers (47 RAPD a...

Assessment of environmental contamination on ecology (plant) at molecular and population levels is important in risk quantification and remediation study. Random amplified polymorphic DNA (RAPD) assay and related other fingerprinting techniques have been employed to detect the genotoxin-induced DNA damage and mutations. This research compared the effects occurring at molecular and population le...

RAPD is a PCR based technique which involves the use of single arbitrary. Advantages disadvantages, limitations and applications of RAPD.RAPD is a modification of the PCR in which a single, short and arbitrary. Therefore, RAPD technique can be performed in a moderate laboratory for most of.sophisticated equipment has made the RAPD technique valuable although. Tartflmann odak noktas ise de, RAPD...