Surface Enhanced Raman Scattering, Hydrogen Exchange, Nucleic Acids The enhanced Raman effect from 5'-AMP adsorbed on the roughened silver surface is used to characterize hydrogen-deuterium exchange. SERS spectra of 5'-AMP present in HzO and D20 solutions at rather low concentrations were recorded in the spectral range of 2000 to 3500 cm-1. Surface enhanced Raman bands in this spectral range ar...

In this study, we present a BCC detector based on confocal Raman spectroscopy using maximum a posteriori probability (MAP) and fuzzy algorithm. The preprocessing step consists of data normalization with minmax method and dimension reduction with principal components analysis (PCA). To enhance the classification performance, we divide Raman spectra into two significant protein bands, i.e., Amid ...

Using the spectral moments method, we present calculations of Raman active modes of Single Walled Boron Nitride Nanotube (SW-BNNT). The Spectra are computed for chiral and achiral nanotubes in terms of their diameter and length. The behaviors of low frequency Raman active modes characteristic, in terms of the tube diameter revealed that these frequencies are diameter dependent. We show that the...

We present preliminary results of Raman scattering measurements of multiwall carbon nanotubes (MWCNT). The nanotubes have been carefully dissolved, separated and then characterized by AFM. The micro-Raman spectra of the objects are taken with 514,5nm wavelength excitations and in the temperature range 4K 400K. Basically the spectra are quite similar to the well known single wall carbon nanotube...

In this work, we utilize a short-wavelength, 532-nm picosecond pulsed laser coupled with a time-gated complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) detector to acquire Raman spectra of several drugs of interest. With this approach, we are able to reveal previously unseen Raman features and suppress the fluorescence background of these drugs. Compared to tra...

Overview: University of Hawaii (UH) in collaboration with NASA Langley Research Center has developed a compact portable sensor platform capable of performing remote Raman, LIBS, Fluorescence and lidar measurements from stand-off distances. The system using a 2.5 inch diameter telescope is capable of measuring Raman, LIBS, and fluorescence spectra of a mineral target from a distance of several m...

UV Raman spectroscopy is a powerful tool for investigating the structures and interactions of the aromatic side chains of Phe, Tyr, Trp, and His in proteins. This is because Raman bands of aromatic ring vibrations are selectively enhanced with UV excitation, and intensities and wavenumbers of Raman bands sensitively reflect structures and interactions. Interpretation of protein Raman spectra ...

Escherichia coli bacteria in the logarithmic growth phase have been investigated by UV resonance Raman spectroscopy. Bacterial whole-cell Raman spectra excited at 251 nm reflect nearly exclusively the nucleic acid composition even though a very large fraction of the bacterial mass is composed of protein. It has been demonstrated that if bacteria are grown under controlled (logarithmic growth) c...

Rapid identification of microbial pathogens reduces infection-related morbidity and mortality of hospitalized patients. Raman spectra and Fourier transform infrared (IR) spectra constitute highly specific spectroscopic fingerprints of microorganisms by which they can be identified. Little biomass is required, so that spectra of microcolonies can be obtained. A prospective clinical study was car...

The potential of laser tweezers Raman spectroscopy (LTRS) to study complex and dynamic cellular processes was investigated on the model of single E. coli cells lysed (1) from "outside" with egg white lysozyme and (2) from "within" by temperature-induced temperate bacteriophage lambdacI857. The two lysis processes differed in the final outcome (incomplete vs complete cell lysis) as revealed by t...