Intrinsically disordered regions (IDRs) play important roles in proteins that regulate gene expression. A prominent example is the intracellular domain of the Notch receptor (NICD), which regulates the transcription of Notch-responsive genes. The NICD sequence includes an intrinsically disordered RAM region and a conserved ankyrin (ANK) domain. The 111-residue RAM region mediates bivalent inter...

The root apical meristem (RAM) determines both RAM activity and the growth of roots. Plant roots are constantly exposed to adverse environmental stresses that can cause DNA damage or cell cycle arrest in the RAM; however, the mechanism linking root meristematic activity and RAM size to the DNA damage response (DDR) is unclear. Here, we demonstrate that a loss of function in RCC1/UVR8/GEF-Like 3...

Milena de Sousa Nascimento, Viviane Gianluppi Ferro & Ricardo Ferreira Monteiro Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Ecologia, Laboratório de Ecologia de Insetos, CP 68020, 21941-590, Rio de Janeiro, RJ, Brazil. Universidade Federal de Goiás, Instituto de Ciências Biológicas, Departamento de Ecologia, 74690-900, Goiânia, GO, Brazil. Corresponding author...

BACKGROUND Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting biomass growth and recombinant-protein production. With the aim to improve mixing and aeration in shake flask cultures, we analyzed cultures subjected to OM and the novel resonant...

AbstPactAhfe RAM is RAM which has been made as reliable as a disk. W e coI1sider the problem of buffer management in partially safe buffers, i.e., butlers which contain both safe RAM and volatile RAM. Buffer managemnt techniques for partially safe buffers explicitly consider the safety of memory in deciding which data to place in the buffer, where to place it, and when to copy updaks back to th...

Postembryonic development of plants depends on the activity of apical meristems established during embryogenesis. The shoot apical meristem (SAM) and the root apical meristem (RAM) have similar but distinct cellular organization. Arabidopsis FASCIATA1 (FAS1) and FAS2 genes maintain the cellular and functional organization of both SAM and RAM, and FAS gene products are subunits of the Arabidopsi...

The application of first-principles computational methods to the study of ferroelectric perovskites has greatly expanded our theoretical understanding of this important class of materials. These methods have most recently been applied to an increasingly wide range of ferroelectric materials, with special attention to their lattice dynamics, phase transitions, and dielectric and piezoelectric pr...