Rat liver L-type pyruvate kinase mRNA was enriched from total polysomes by immunoprecipitation with a specific antibody and Staphylococcus aureus cells. Double-stranded cDNA synthesized from the enriched mRNA was inserted into the PstI site of pBR322, and the resultant recombinant DNA molecules were used to transform Escherichia coli. Three clones containing DNA complementary to L-type pyruvate...

Hepatocytes were isolated from preweaned neonatal and adult rats and maintained in primary monolayer culture. Cells from preweaned newborns possessed no L-type pyruvate kinase, nor did they synthesize the enzyme. Incubation for 48-72 h in culture medium supplemented with 2 mM-fructose and 0.1 microM-insulin induced the synthesis of L-type pyruvate kinase, as judged by increased enzyme activity ...

The relationship between liver (L-type) and erythrocyte pyruvate kinase remains a subject of discussion. It has been previously demonstrated that these enzymes are kinetically [ 1,2] and immunologltally [3-51 related. In some cases liver (Ltype) pyruvate kinase from patients with hereditary erythrocyte pyruvate kinase deficiency was shown to be defective [2,6-81. Erythrocyte and L-type enzymes,...