In connection with a study of the ultracentrifugal sedimentation of antibody preparations (1) the opportunity arose for study of the electrophoretic properties of some of the highly active material under investigation. Measurements of this kind are of value in the characterization of proteins and similar high molecular substances, and may also give information regarding the chemical homogeneity...

Glycogen phosphorylase has been purified from rabbit liver by a simplified procedure which depends on the initial separation of the enzyme from a crude extract by centrifuging it as a complex with particulate glycogen. Initial rates have been measured with varied concentrations of both substrates in each reaction direction. The data were analyzed with double reciprocal plots and secondary plots...

The structure of purified preparations of activated (DNA-binding) glucocorticoid receptor (GR) was analyzed in the presence or absence of DNA. A 35-base pair DNA fragment harboring a strong GR-binding site from the mouse mammary tumor virus promoter (-189/-166) was used for stoichiometric analysis of the GR . DNA complex. Glycerol gradient centrifugation was utilized in order to separate the 6 ...

In connection with a study of the ultracentrifugal sedimentation of antibody preparations (1) the opportunity arose for study of the electrophoretic properties of some of the highly active material under investigation. Measurements of this kind are of value in the characterization of proteins and similar high molecular substances, and may also give information regarding the chemical homogeneity...