Psychrophilic methanogenic Archaea contribute significantly to global methane emissions, but archaeal cold adaptation mechanisms remain poorly understood. Hinted by that mRNA architecture determined secondary structure respond to cold more promptly than proteins, differential RNA-seq was used in this work to examine the genome-wide transcription start sites (TSSs) of the psychrophilic methanoge...

The cloning, overexpression and characterization of a cold-adapted DNA ligase from the Antarctic sea water bacterium Pseudoalteromonas haloplanktis are described. Protein sequence analysis revealed that the cold-adapted Ph DNA ligase shows a significant level of sequence similarity to other NAD+-dependent DNA ligases and contains several previously described sequence motifs. Also, a decreased l...

background: organic agricultural pesticides are so stable in ecosystems and biosphere. these compounds affect soil conditions and agricultural products. the aim of this study was isolation of diazinon-degrading bacteria from kerman pistachio orchards, southeastern iran. methods: diazinon-degrading bacteria were enriched in bushnell-hass medium. identification and sequencing of prevalent degradi...

abstract background: bioaerosol is defined as airborne particles such as living organisms include bacteria, fungi and their related metabolites, such as endotoxin. over the recent years, health effects of bioaerosols such as infectious and respiratory diseases, acute toxic effects, allergies and cancer have been considered. the aim of this study was to evaluate the bacterial and fungal concentr...

conclusions the ppi-g2 dendrimer with end amine groups exhibited a positive impact on the removal of dominant bacterial isolated strains. therefore, it is possible to use these nanodendrimers as a safe and effective material for water disinfection in the future. results the most important isolated bacteria were escherichia coli 37 (48.7%), klebsiella oxytoca 12 (15.8%), proteus mirabilis 3 (4%)...

infectious diseases are one of the most common causes of morbidity and mortality and the spread of resistant microorganisms is playing a significant role in this regard. the purpose of this study was to assess the trend in antimicrobial resistance of gram-positive bacteria at the main referral teaching hospital in tehran during a 4-year period. all patients' biological isolates such as blood, u...

the performance of selective media ltb, (leauryl tryptose broth), mmgm (minerals modified glutamate medium), bgbb (brilliant green bile broth), for the detection of e.coli in water in water, were compared and the following result were obtained: the mmgm found to be unsuitable as confirmatory medium for the detection of e.coli at 44 c, due to the occurrence of an unacceptable number of the false...

nowadays the most difficult problem in treatment of bacterial infections is the appearance of resistant bacteria to the antimicrobial agents so that the attention is being drawn to other potential targets. in view of the positive findings of phage therapy, many advantages have been mentioned which utilizes phage therapy over chemotherapy and it seems to be a promising agent to replace the antib...

background: to determine prospectively the prevalence of anaerobic and aerobic infection in early onset (during 72 hours of age) neonatal sepsis, in tehran vali-e-asr hospital. methods: among all the live birth, neonates suspecting of having septicemia were investigated for isolation of micro­organisms. culture bottle containing enriched tryptic soy broth was used for standard blood culture sys...