Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous species were infected with cauliflower mosaic virus; they include Moricandia arvensis, a plant not k n o w n to be susceptible. With this technique > 90~o of turnip protoplasts were infected, each producing an average of 104 to 105 viral genome equivalents. Filter hybridization and fluorescent antibod...

Rosner, Richard (St. Joseph's Hospital, Paterson, N.J.). Isolation of Candida protoplasts from a case of Candida endocarditis. J. Bacteriol. 91:1320-1326. 1966.-A case of endocarditis caused by Candida tropicalis is described. Even though the patient was receiving adequate therapy, and all routine blood cultures were negative for growth, the patient continued to give clinical evidence of active...

Plant protoplasts are significant for plant cell culture, somatic fusion, genetics, and breeding studies. In addition, in vitro regeneration has great importance developmental biology, manifesting potential applications agriculture biotechnology. this regard, we present a well-established protocol regarding protoplast isolation, culture fusion of Jasminum spp. particular, different tissues sama...

The uptake of lucifer yellow CH by suspension-cultured carrot cells and protoplasts has been studied by laser scanning microscopy. This fluorochrome, which does not diffuse across membranes, gradually accumulates in the cell vacuole over a period of hours. In contrast, the central vacuole of protoplasts did not show lucifer yellow fluorescence. The latter was restricted, in protoplasts, to punc...

Tobacco mosaic virus (TMV) RNA was encapsulated in large, unilamellar phospholipid vesicles (liposomes), and the encapsulated TMV RNA was shown to be infectious when incubated with tobacco protoplasts under appropriate conditions. Maximal virus production in protoplasts was observed after their incubation with TMV RNA entrapped in phosphatidylserine/cholesterol liposomes. Infection was dependen...

Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...

Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Intr...

Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/ mL), in an osmotic solution for 90 min at 28oC. The frequency of regenerated pro...

A vena sativa , Chloroplasts, Protoplasts, Sulfite Efflux, Sulfite Uptake Processes of J5S-labelled sulfite uptake and efflux by oat protoplasts and their chloroplasts are very rapid: during the first minutes (or perhaps even seconds) after sulfite supply a state of equilibrium is established between the protoplasts and their chloroplasts and the incubation medium. Bicarbonate concentrations ra...

PREMISE OF THE STUDY Understanding plant cell biomechanics has been hampered by a lack of appropriate experimental tools. Here we introduce a protocol for the incorporation of individual plant protoplasts into precisely sized agarose microbeads. This technology may lead to new ways to manipulate the physical and chemical microenvironment of individual plant cells. METHODS AND RESULTS Living p...