Anthropogenic and extreme climate disasters cause ecological changes in natural rivers lakes, increasing the risk of starvation yellowcheek (Elopichthys bambusa). Therefore, impact on metabolism wild population should be explored. In this study, we used transcriptome sequencing technology to analyze effects short (8 d) long-term (28 liver transcriptome, growth, serum indicators yellowcheek. Our...

The phoA gene fusion methodology permitted the identification of a new Mycobacterium tuberculosis exported protein, Des. This protein has significant sequence similarities to plant acyl-acyl carrier protein desaturases, which are enzymes involved in general fatty acid biosynthesis as well as in mycolic acid biosynthesis in mycobacteria. As shown by Western blot and enzyme-linked immunosorbent a...

the development of reliable processes for the synthesis of nanoparticles is an important aspect of nanotechnology. biologically synthesized nanoparticles could have innumerable applications in different areas such as reception, catalyzers, biolabellers, etc. in the present review, we emphasize the richness of the microbial world which encompasses a plethora of endophytic entities as an emerging...

The biotrophic fungus Ustilago maydis causes smut disease in maize with characteristic tumor formation and anthocyanin induction. Here, we show that anthocyanin biosynthesis is induced by the virulence promoting secreted effector protein Tin2. Tin2 protein functions inside plant cells where it interacts with maize protein kinase ZmTTK1. Tin2 masks a ubiquitin-proteasome degradation motif in ZmT...

It was found that the partially purified beta-ketoacyl-ACP synthase of Bacillus insolitus did not require the addition of FabD (malonyl-CoA:ACP transacylase, MAT) for the activity assay. This study therefore examined the necessity of FabD protein for in vitro branched-chain fatty acid (BCFA) biosynthesis by crude fatty acid synthetases (FAS) of Bacilli. To discover the involvement of FabD in th...

BACKGROUND Chemical-genetic profiling of inhibitory compounds can lead to identification of their modes of action. These profiles can help elucidate the complex interactions between small bioactive compounds and the cell machinery, and explain putative gene function(s). RESULTS Colony size reduction was used to investigate the chemical-genetic profile of cycloheximide, 3-amino-1,2,4-triazole,...