Serum protein electrophoresis (SPE) is the hub of laboratory testing for monoclonal gammopathies and is a component of almost all diagnostic panels (1–3 ). The sensitivity of SPE as a screening test, however, is dependent on how SPE abnormalities are defined (4 ). Discrete, quantifiable electrophoretic bands (M-spikes) are almost always monoclonal proteins. Subtle abnormalities such as fuzzy ba...

 This study was aimed to detect anti-Toxoplasma gondii antibodies in cats infected with Rh strain of T. gondii by using an immunoblotting method. For this, cats were experimentally infected using tachyzoites harvested from Vero cell cultures. Tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to PVDF membranes. An immunoblotting was performed using these mem...

Sparse Raman-based models can reach high accuracy and stability for the prediction of fine biological processes (immune activation protein synthesis), privilege use side bands discriminating cellular differences.

background: to use different methods for serodiagnosis of ruminants' haemonchosis is important because detection of egg in the faeces is not so reliable. methods: peptide bands of 5 different crude antigens of intestine, uterus, cuticle, whole male and whole female of hae­monchus contortus were determined using sds-page and western blotting. five lambs were infected with 10,000 third stage larv...

Protein profiles of selected Salmonella serovars were compared with E. coli to identify genus specific protein(s) for Salmonella. The PDP formed of different Salmonella serovars were compared with E. coli O78 when subjected to SDS-PAGE yielded 11, 15, 15, 11 and 14 bands in S. Bareilly, S. Gallinarum, S. Typhimurium and S. Weltevreden and E. coli O78 respectively. The bands produced were compar...

In present investigation, the protein and polypeptide profiles of some pigeonpea mutants have been analyzed on Native and SDS-PAGE to understand genetic variability .Native PAGE gel analysis of viable mutants in variety BDN 708 showed considerable variations in protein profiling. SDS-PAGE analysis of viable mutants of pigeonpea revealed a wide range of protein polymorphism and variability with ...

Desmosomes, isolated from cow nose epidermis by a method utilizing citrate buffer pH 2.6 and density gradient centrifugation, have been analyzed and found to contain approximately 76% protein, 17% carbohydrate, and 10% lipid. Nonpolar amino acids predominate in desmosomal protein, representing 456 residues per 1,000. The sialic acid content is 5 nM/mg of protein. The lipid fraction is composed ...

background: candida species are among the most common causes of opportunistic fungal diseases. among candida species, candida albicans is responsible for most infections. having many strains, c. albicans is very polymorph. c. dubliniensis is very similar to albicans species both morphologically and physiologically. for an infection to occur, cell wall proteins play an important role as they ena...