Alkaline proteases are the most important groups of commercial enzymes, which have been broadly used in industrial processes. In this study, Bacillus sp. PTCC 1538 was selected as a biological agent to produce alkaline protease. Enzyme production under submerge fermentation using waste effluent investigated. Since costs raw material plays an role cost enzyme production, corn steep liquor (CSL) ...

In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which three factors including alginate type, calcium chloride concentration, and agita...

The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production ...

In this work the subC gene from Bacillus licheniformis encoding subtilisin was cloned into the nisin-controlled expression (NICE) vectors (pNZ8048 and pNZ8148) with or without the signal peptide SP Usp45 directing extracellular secretion via Sec machinery. Extracellular protease production and activity was tested using Lactococcus lactis NZ9000 as host, which could be used for rennet production...

Protease production by Bacillus subtilis with free and immobilized cells was examined in this study. Entrapment method of immobilization was used with gelatin, polyacrylamide, calcium alginate and agar matrices and protease production was studied compared to equivalent weight of free cells. Results showed that the gelatin entrapped cells produce 10.8 U/ml; the maximum enzyme titer followed by p...

previous studies had shown that sub-inhibitory concentrations of silver sulphadiazine (agsd) stimulated the production of toxic shock syndrome toxin-1 in certain strains (responder strains) of staphylococcus aureus and that protease production was also affected. no changes were detected in other strains (non-responders). extracellular proteins from eleven responder and non-responder strains gro...