Human platelets that had been preincubated with 5-hydroxy[(3)H]tryptamine and [(32)P]P(i) were stirred with various agents; the secretion of 5-hydroxy[(3)H]tryptamine from platelet granules and the radioactivity of platelet [(32)P]phosphopolypeptides separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis were then measured. Exposure of the platelets to collagen fibres or ionoph...

PURPOSE To investigate the alterations in gene expression when human retinal pigment epithelial (RPE) cells in culture are treated with vitreous as a model for the changes that occur in proliferative vitreoretinopathy. METHODS Human RPE cells were cultured with or without human vitreous or collagen. RNA was extracted and reverse transcribed. The RNAs expressed were compared by using DNA macro...

The synthesis of prostanoids by the Sertoli cell was assessed as part of a study on the role of vitamin E in maintaining spermatogenesis. Analyses of eicosanoid synthesis from endogenous substrate were carried out using freshly isolated Sertoli-cell-enriched preparations from both pre-pubertal and adult rats fed purified diets with and without vitamin E, as well as cells carried in primary cult...

OBJECTIVE To explore the mechanism by which diclofenac sodium eyedrops exert an adverse effect on the cornea. METHODS In 10 healthy Japanese volunteers, 0.1% diclofenac sodium solution was instilled into one eye 3 times daily for 2 weeks. Only vehicle was applied to the other eye. Tear samples were taken before drug treatment, at 2 weeks (on the final day of treatment), and at 4 weeks. Prosta...

features of chronic periodontal disease and it has been demonstrated (Goodson et al., 1974; El Attar, 1976) that inflamed gingivae contain much higher concentrations of prostaglandin E, than normal tissue. Chronically inflamed gingivae are infiltrated with large number of macrophages, lymphocytes and plasma cells, which interact in the immune response. Products of these cell interactions may mo...

Matrix metalloproteinase (MMP)-9 contributes to the pathogenesis of chronic inflammatory diseases and cancer. Thus, identifying targetable components of signaling pathways that regulate MMP-9 expression may have broad therapeutic implications. Our previous studies revealed a nexus between metalloproteinases and prostanoids whereby MMP-1 and MMP-3, commonly found in inflammatory and neoplastic f...

A number of studies have identified cytosolic prostaglandin E(2) synthase (cPGES)/p23 as a cytoplasmic protein capable of metabolism of prostaglandin E(2) (PGE(2)) from the cyclooxygenase metabolite prostaglandin endoperoxide (PGH(2)). However, this protein has also been implicated in a number of other pathways, including stabilization of the glucocorticoid receptor (GR) complex. To define the ...

References 1. The ICAI ST U DY GRO U P: P rostanoids fo r chronic critical leg ischemia. A randomized, controlled, open-label trial with Prostaglandin E1. Ann Intern Med 1999; 130: 412-21. 2. MARTIN MFR, TOOKE JE: Effects of Prostaglandin E1 on microvascular haemodynamics in progressive systemic sclerosis. Br Med J 1982; 285: 1688-90. 3. YO S H I K AWA T, S U Z U K I H , K ATO H , YA N O S: Eff...