the purpose of this study was to examine the effectiveness of nanosilver colloid as a new antimicrobial agent against erwinia in tissue culture of sugar beet for the recovery of the contaminated doubled haploid plantlets. the nanosilver colloidal solution of 4000 ppm was diluted in different concentrations (0, 50, 75, 100 and 125 ppm) and was added to the solid ms medium. in a factorial experim...

A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environment. The greenhouse and field have substantially lower relative humidity, higher light level and septic environment that are stressful to micropropagated plants compared to in vitro conditions. The benefit of any micropropagation system can, however, only be fully reali...

Garlic (Allium sativum) is propagated asexually. Since sexual cross breeding is almost impossible, means for effective breeding are not currently available and the available production cultivars are seriously aged and degenerated. A possible alternative for breeding is chemical induction. Trifluralin, a type of herbicide, has been reported to provoke chromosome doubling. However, this che...

Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 μ M BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 μ M BA and 0.05 μ M NAA. On average, 3.6 shoots per explant were obtained. Four weeks o...

We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no esca...

Title of Thesis: Clustering with Propagated Constraints Eric Robert Eaton, Master of Science, 2005 Thesis directed by: Dr. Marie desJardins, Assistant Professor Department of Computer Science and Electrical Engineering Background knowledge in the form of constraints can dramatically improve the quality of generated clustering models. In constrained clustering, these constraints typically specif...

The growth of strawberry plantlets in the rooting stage on culture medium supplemented with silver nanoparticles (AgNPs) and ethylene gas accumulation plantlet bottles were investigated. In addition, different systems first used to produce large-scale Strawberry plantlets. results showed that shoots (3 cm) cultured MS 0.02 mg/L NAA, 1 g/L activated charcoal, 30 sucrose, 8 agar 0.5 AgNPs about 4...

Using cotyledons of 2-week old in vitro germinated seedlings, a rapid and efficient regeneration protocol was established for A. mangium on Murashige and Skoog (MS) basal medium. Cotyledons showed better direct shoot organogenesis producing maximum 10.88 ± 0.35 shoots per explant when cultured on MS medium supplemented with 2.0 μM BA and 1.0 μM NAA. Full strength of MS medium supplemented with ...