BACKGROUND We investigated the species distribution and amphotericin B (AMB) susceptibility of Korean clinical Aspergillus isolates by using two Etests and the CLSI broth microdilution method. METHODS A total of 136 Aspergillus isolates obtained from 11 university hospitals were identified by sequencing the internal transcribed spacer (ITS) and β-tubulin genomic regions. Minimal inhibitory co...

Three sections of Aspergillus (five species, 21 strains) were classified according to culture medium-dependent and time-dependent secondary metabolite profile-based chemotaxonomy. Secondary metabolites were analysed by liquid chromatography-electrospray ionisation tandem mass spectrometry (LC-ESI-MS-MS) and multivariate statistical methods. From the Aspergillus sections that were cultured on ma...

Platycodon grandiflorum A. DC (Campanulaceae) is a traditional medicinal plant. Its root, Platycodi Radix, contains an abundant amount of saponin glycosides, platycodins, of which platycodin D is one of the major components. The chemical structures of platycodins can be modified by various types of chemical processing, but a modification mediated with microorganisms has been not reported yet. I...

BACKGROUND meso-Galactaric acid is a dicarboxylic acid that can be produced by the oxidation of D-galacturonic acid, the main constituent of pectin. Mould strains can be engineered to perform this oxidation by expressing the bacterial enzyme uronate dehydrogenase. In addition, the endogenous pathway for D-galacturonic acid catabolism has to be inactivated. The filamentous fungus Aspergillus nig...

The low fiber digestibility and unbalanced amino acids restricted the use of DDGS in swine diets. Key nutrition components dynamic monitoring key regulatory pathways analysis were performed to find rules changes for fermented by Aspergillus niger. Cellulose hemicellulose reduced 15.3% 15.2%. 1,4-D-Xylobiose was decreased from 16.8 ?g/mL 0.2 ?g/mL. Lys, Arg, Thr increased 3.00%, 2.89%, 4.40%, me...

Aspergillus niger van Tieghem, Aspergillus tamarii Kita, and Aspergillus flavus Link ex Fries utilized the methylthio moiety of 2,4-bis(isopropylamino) -6-methyl-mercapto-s-triazine (prometryne) as a sulfur nutrient source. Other soil fungal isolates not affected by prometryne concentrations to 1 mg/ml culture included: Aspergillus oryzae (Ahlburg) Cohn, Curvularia lunata (Wakker) Boedijn, Tric...

We report the cloning and characterization of a gene encoding a ferulic acid esterase, faeA, from Aspergillus niger and Aspergillus tubingensis. The A. niger and A. tubingensis genes have a high degree of sequence identity and contain one conserved intron. The gene product, FAEA, was overexpressed in wild-type A. tubingensis and a protease-deficient A. niger mutant. Overexpression of both genes...

Biodiversity of phylloplane and endophytic fungi have been considered as one of the rich source of novel natural compounds of biological activities and a high level of structure diversity on the leaf surfaces. Bioactive components produced by phylloplane and endophytes have shown promising potentiality towards human health. In this juncture, it is necessary to understand and manipulate this imp...