Sugarcane mosaic disease is caused by the Sugarcane streak mosaic virus (SCSMV; genus Poacevirus, family Potyviridae) which is common in some Asian countries. Here, we established a protocol of a one-step real-time quantitative reverse transcription PCR (real-time qRT-PCR) using the TaqMan probe for the detection of SCSMV in sugarcane. Primers and probes were designed within the conserved regio...

Triticum mosaic virus (TriMV) (genus Poacevirus, family Potyviridae) is a recently described eriophyid mite-transmitted wheat virus. In vitro RNA transcripts generated from full-length cDNA clones of TriMV proved infectious on wheat. Wheat seedlings inoculated with in vitro transcripts elicited mosaic and mottling symptoms similar to the wild-type virus, and the progeny virus was efficiently tr...

The multifunctional nuclear inclusion protein a (NIa) of potyviruses (genus Potyvirus; Potyviridae) accumulates in the nucleus of virus-infected cells for unknown reasons. In this study, two regions in the viral genome-linked protein (VPg) domain of NIa in Potato virus A (PVA) were found to constitute nuclear and nucleolar localization signals (NLS) in plant cells (Nicotiana spp). Amino acid su...

Previously, we reported that coat protein (CP) of Wheat streak mosaic virus (WSMV) (genus Tritimovirus, family Potyviridae) tolerates deletion of amino acids 36 to 84 for efficient systemic infection of wheat. In this study, we demonstrated that WSMV mutants with deletion of CP amino acids 58 to 84 but not of 36 to 57 induced severe chlorotic streaks and spots, followed by acute chlorosis in wh...

A series of in-frame and nested deletion mutations which progressively removed 5'-proximal sequences of the Wheat streak mosaic virus (WSMV) HC-Pro coding region (1152 nucleotides) was constructed and evaluated for pathogenicity to wheat. WSMV HC-Pro mutants with 5'-proximal deletions of 12 to 720 nucleotides systemically infected wheat. Boundary sequences flanking the deletions were stable and...

Potato Virus Y (PVY) is a member of the Potyviridae family – the largest plant virus family. PVY infects Solanaceae – agriculturally important species such as tobacco, potato, pepper, tomato This pathogen causes decrease in yield and quality. PVY isolates have been divided into three main strains: PVYN, PVYO and PVYC, according to the symptoms induced on tobacco and potato. PVYN isolates can be...

Triticum mosaic virus (TriMV), the type member of the newly proposed Poacevirus genus, and Wheat streak mosaic virus (WSMV), the type member of Tritimovirus genus of the family Potyviridae, infect wheat naturally in the Great Plains and are transmitted by wheat curl mites. In this study, we examined the ability of these viruses to infect selected cereal hosts, and found several differential hos...

turnip mosaic virus (tumv) is amember of the potyvirus genus within the potyviridae family and is one the most important viruses infecting brassicaceae plants. in april 2012, suspicious symptoms of a viral disease such as mosaic, stunting and malformation were observed on herschfeldia incana. the collected samples were tested using reverse transcription-polymerase chain reaction (rt-pcr) with s...

plum pox virus (ppv), the causal agent of sharka disease, belongs to the genus potyvirus in the family potyviridae and has been reported from some regions of iran. surveys were conducted to determine the incidence of the virus in khorasan razavi province in 2010 and 2011 and 553 samples of stone fruit trees were collected and tested by das-elisa. in this method 12 samples were infected with ppv...

The Cowpea aphid-borne mosaic virus (CABMV), of the genus Potyvirus (Potyviridae), causes most prominent passion fruit (Passiflora spp.) disease in Brazil. is transmitted a non-circulating manner by many species aphids. Chemical control vector insect inefficient, due to nature virus-vector aphid relationship, and commercial are susceptible virus. As such, comprehending disease’s spatio-temporal...