We have determined the number of proteins in rat skeletal muscle 80 S ribosomes and ribosomal subparticles by twodimensional polyacrylamide gel electrophoresis. The proteins were compared with those from liver ribosomes. The small subunit of muscle ribosomes contained 31 proteins: it had two proteins (SJ and S,,,2) which were not found in liver 40 S particles; one protein (S31*) was always foun...

Synthetic cannabinoids cause a variety of central nervous symptoms, tachycardia and hypokalemia. We report a 27 old male patient was admitted to emergency room due to altered consciousness and vomiting. The patient declared that he smoked herbal incense named Bonzai. After a few hours he was found by his friends with impaired consciousness, no verbal response, and tonic seizure of upper extremi...

Several factors interact to maintain precise control of electrolyte transport in the mammalian cortical collecting duct. We have studied the effects of deoxycorticosterone, arginine vasopressin, and bradykinin on net transepithelial sodium and potassium transport in isolated, perfused rat cortical collecting ducts. Chronic administration of deoxycorticosterone to rats increased both sodium abso...

L cells have a resting potential of about -16 mv (internal negative) at 37 degrees C in Dulbecco's modified Eagle's medium containing 10% fetal calf serum and a potassium concentration of 5.4 mM. Membrane resistivity is about 20,000 ohmcm(2) when the surface filopodia described by others are taken into account. Mechanical and electrical stimuli can evoke an active response from mouse L cells, c...

THE EFFECT OF EXPOSING RABBIT RETICULOCYTE RIBOSOMES TO CONCENTRATED SOLUTIONS OF POTASSIUM CHLORIDE WAS EXAMINED BY: (a) dialysis against 0.5m-potassium chloride; (b) zone centrifugation through a sucrose gradient in 0.5m-potassium chloride; (c) differential centrifugation of a solution made 0.5m with respect to potassium chloride. The products of each treatment and their ability to support pr...

Whole gut perfusion in humans was used to compare the effect on intestinal water and electrolyte transport of the World Health Organisation oral rehydration solution (solution II, composition in mmol/l: glucose 111, sodium 90, bicarbonate 30, potassium 20; 308 mOsm/kg); a hypertonic commercial oral rehydration solution (solution III, glucose 188, sodium 50, bicarbonate 20, potassium 20 mmol/l; ...

Introduction: Alzheimer’s disease is a progressive neurodegenerative disorder, characterized by impairment of memory and changes in behavior and personality. Recent evidence suggests that mitochondrial channels play important roles in memory disorders. Accordingly, the biophysical properties of a single potassium channel were investigated in the brain mitochondrial inner membrane of rat with...

The ROMK family of proteins has biophysical properties and distribution within the kidney similar to those of secretory potassium channels of the distal nephron. To study the regulation of ROMK during variations in dietary potassium, we measured the abundance of ROMK protein in rat kidney by immunoblotting. Neither 2 nor 5 days of a high-potassium diet had an effect on protein abundance in the ...

A simple, specific, accurate, and precise reverse phase high-performance liquid chromatographic method for analysis of Cefuroxime axetil and Potassium clavulanate has been developed. Separation of drugs was carried out on Jasco HPLC system with Hypersil Gold C-18 column (250 mm × 4.6 mm i.d.), using 0.01M Potassium dihydrogen phosphate: methanol 60: 40 (v/v) as mobile phase. Quantitation of dru...

Previous studies have shown that administration of rubidium to normal or potassium-depleted rats results in a) metabolic acidosis (1), and b) accumulation of rubidium in muscle tissue where it attains final concentrations considerably higher than that of potassium (2). Since it was found that rubidium was at least as effective as potassium in lowering the plasma CO2 content of potassium-deficie...