The freezability of semen collected during the low (May-July) and the peak (September-November) breeding seasons from 18 Nili-Ravi buffalo bulls of young (3-4 years), adult (6-8 years) and old ( 1215 years) age groups was studied. After extension in lactose.:..fructose-egg-yolk-glycerol extender, semen samples were frozen and stored in liquid nitrogen. Post-thaw motili�y and liveability of sper...

In the first experiment, osmotic pressure of semen and seminal plasma in a semen sample from each of the 20 mature Nili-Ravi buffalo bulls was determined. In the second experiment, effects of osmotic pressure on motility (%), plasma membrane integrity (%) and viability (%) in fresh and frozen-thawed semen samples from each of the seven mature Nili-Ravi buffalo bulls was determined. In the first...

Extended semen from six Hereford bulls was placed in .25-ml Continental straws and frozen in the vertical position. Treatments were arranged factorially with glycerol levels of 5, 7, 9 or 11%; semen cooled from 5 to 1 3 0 C in 3.5 (fast), 20 (moderate) or 40 min (slow); and thawed in water at 5 C for 3 min, 35 C for 12 sec, 55 C for 8 sec, 75 C for 7 sec or 90 C for 5 seconds. Fast freezing res...

The present study was undertaken to examine the viability, motility and morphology of spermatozoa that were frozen-thawed at different temperature followed by the storage at 4oC until 10 hours under the condition of tropical area. The frozen semen derived from a Holstein-Friesian bull with the egg-yolkbase extender was thawed at 23oC or 37oC for 30 seconds or 15 seconds, respectively, and kept ...

objective: the aim of this study was to select the best medium to maintain sperm motility during sperm-dna incubation and assess the dna uptake by spermatozoa of iranian holstein bulls and its effects on sperm motility. materials and methods: frozen sperms from an iranian holstein bull were thawed and centrifuged. motile sperms were separated through puresperm gradient (40/80%) followed by two ...

This study evaluated the effects of superoxide dismutase (SOD) on viability and acrosome integrity of European mouflon spermatozoa after cryopreservation and on the fertilization rates of sheep oocytes after i.v.f. or intracytoplasmatic sperm injection (i.c.s.i.). Frozen semen was thawed and washed with synthetic oviduct fluid supplemented with 0.6% bovine serum albumin. After centrifugation, t...

Cryopreservation of spermatozoa is a pivotal tool in assisted reproduction, and studies aiming to establish optimal freezing/thawing protocols are essential to enhance sperm survival. The objectives of the present study were to (1) compare the cryoprotective efficiency of three different glycerol concentrations (3%, 5%, and 7%) on the basis of post-thaw sperm quality and (2) investigate whether...

Objective: To examine the qualitative and quantitative characteristics of frozen-thawed spermatozoa recovered through the SpermPrep (ZBL, Inc., Lexington, KY) filtration method or the swim-up technique for the purpclse of intrauterine insemination (IUI) or other techniques for assisted reproduction. Design, Setting, Patients: Thirty pairs of frozen specimens purchased from three commercial seme...

The aim of this study was to evaluate the effect of semen collection method (artificial vagina compared to electroejaculation), season in which the semen was collected (breeding season compared to non-breeding season), freezing extender (Biladyl(®), Andromed(®) and skim milk based extender) and pre-treatment procedure (washing compared to non-washing) on post-thaw semen quality in buck. Ejacula...

The principal objective of this study was to derive an improved procedure for cryopreservation of swamp buffalo (Bubalus bubalis) spermatozoa. Experiments were conducted to determine effects of cooling rate, intermediate plunge temperature and warming rate on motility and acrosome integrity of spermatozoa. Spermatozoa were obtained from three bulls (three ejaculates/bull) and were subjected to ...