A major drawback of Amplified Fragment Length Polymorphisms (AFLP) as genetic makers for phylogeographic studies is their lack of a temporal dimension. In a recent publication in Molecular Ecology, Kropf et al. (2009) proposed a molecular clock for AFLP. In this comment we evaluate the proposed approach both theoretically and empirically. A linear increase with time is a prerequisite to use a g...

The fitness of organisms may be due chiefly to a fitness curve imposed on their ranking in the population with respect to heterozygosity. If this is so, then the number of polymorphisms that can be retained at a particular selective equilibrium increases as the square of the population size. All of the genetic variation that we currently observe and infer to exist can probably be maintained by ...

Nucleotide sequence variation at the Acp29AB gene region has been surveyed in Drosophila melanogaster from Spain (12 lines), Ivory Coast (14 lines), and Malawi (13 lines) and in one line of D. simulans. The approximately 1.7-kb region studied encompasses the Acp29AB gene that codes for a male accessory gland protein and its flanking regions. Seventy-seven nucleotide and 8 length polymorphisms w...

Alloimmune specificity and histocompatibility, driven by genetic polymorphism, are ancient determinants of self-/non-self-recognition. Recent molecular genetic evidence has revealed an allodeterminant in the cnidarian Hydractinia that consistently predicts histocompatibility reactions.

In many species, genomic data have revealed pervasive adaptive evolution indicated by the fixation of beneficial alleles. However, when selection pressures are highly variable along a species' range or through time adaptive alleles may persist at intermediate frequencies for long periods. So called "balanced polymorphisms" have long been understood to be an important component of standing genet...

Abstract The plant pathogen Phytophthora capsici can be found all throughout the USA, and population genetics of this organism have been studied within many these states. Until now, no work has done in state Tennessee to investigate structure P. there. was explored using 296 isolates collected from five counties 2004, 2007, 2018, 2019. Samples were genotyped 39 single nucleotide polymorphism (S...

Selection and use of molecular markers for evaluation DNA polymorphism in plants are couple the most important approaches field genetics. The assessment genetic diversity using morphological is not sufficient due to little differentiating traits among species, genera or their individuals. Morphological only highly influenced by environmental factors but skilled also prerequisite find variations...

Microsatellites are the most popular and versatile genetic marker with myriads of applications in population genetics, conservation biology, and evolutionary biology. These are the arrays of DNA sequences, consisting of tandemly repeating mono-, di-, tri-, and tetranucleotide units, which are distributed throughout the genomes of most eukaryotic species. Microsatellites are codominant in nature...

We have developed DNA-based genetic markers for rapid cycling Brassica rapa (RCBr), also known as Fast Plants. Although markers for B. rapa already exist, ours were intentionally designed for use in a teaching laboratory environment. The qualities we selected for were robust amplification in PCR, polymorphism in RCBr strains, and alleles that can be easily resolved in simple agarose slab gels. ...

The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in their principles, methodologies, and applications require careful consideration in choosing one or more of such methods. No molecular markers are ...