Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs cation step adds time, cost, and aff ects the quality of testing. objective this study was to develop a protocol for direct saliva in genetic markers, without acids. Methods. PCR, real-time RT-PCR isothermal amplifi were used detection Results. We report markers ...

The citrus systemic diseases, including Huanglongbing (caused by Candidatus Liberibacter asiaticus (CLas)), tristeza virus (CTV)), tatter leaf (CTLV)), and exocortis viroid (CEVd)), are threats to production in Taiwan. Reliable diagnostic methods important for the management of these diseases. In this study, we developed a multiplex reverse transcription–polymerase chain reaction (RT-PCR) assay...

Objectives: To compare the performance of chest CT and Real-Time Reverse Transcription-Polymerase Chain Reaction (RTPCR) in initial diagnostic assessment coronavirus disease COVID-19 patients during pandemic. Methods& Results:In this study we have included 209 clinically suspected cases COVID-19from Government Medical College, Nagpur who undergone both HRCT RT-PCR. In our study, 3.35% were ...

abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction   (ic-rt-pcr) indicated that pepper isol...

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

Abstract Potato viruses PLRV, PVY, PVM, PVA, PVX and PVS can cause up to 90% loss of potato harvest. Therefore, they are monitored by law in many countries using DAS-ELISA or conventional real-time RT-qPCR. Previously, we developed a multiplex DiRT-PCR (Direct reverse transcript – polymerase chain reaction), which works directly on diluted tuber sap thus saves time chemical processing for RNA e...

The coronavirus, which appeared in Wuhan city of China and named COVID-19 , spread rapidly caused the death many people. Early diagnosis is very important to prevent or slow spread. first preferred method by clinicians real-time reverse transcription-polymerase chain reaction (RT-PCR). However, expected accuracy values cannot be obtained patients incubation period. Therefore, common lung devast...

RAF1 (c-raf) has been known as an important tumor-promoter in many cancers. However, the regulatory mechanisms affecting expressions are rarely reported. This study aimed to predict candidate miRNAs of gene and verify their functions progression lung cancer. The expression miR-643 was detected by reverse transcription polymerase chain reaction (RT-PCR). A dual-luciferase report system used rela...