BACKGROUND Matairesinol is a plant lignan present in a wide variety of foodstuffs such as seeds, vegetables and fruits. It has various biological functions including anti-angiogenic, anti-cancer and anti-fungal activities, but its anti-osteoporotic activity, if any, is unknown. METHODS For osteoclast differentiation, primary mouse bone marrow-derived macrophage cells (BMMs) were cultured for ...

Retroviral gene transfer into human myeloid precursor cells allows introduction of marker genes as well as genes conferring resistance to chemotherapeutic drugs. We transduced a human mutant dihydrofolate reductase (DHFR) cDNA into CD34 antigen-positive peripheral blood cells from patients with breast or ovarian cancer obtained after treatment with chemotherapy and granulocyte colony-stimulatin...

Chylomicron remnants transport cholesterol from the intestine, and are removed from the circulation principally by the liver. While hepatic receptors, including the low density lipoprotein (LDL) receptor account for endocytosis, heparan sulfate proteoglycans (HSPG) participate in the initial binding of remnants to liver cells. To explore the interactions between HSPG and endocytosis of remnants...

BACKGROUND The transcription factor GATA-2 is predominantly expressed in hematopoietic stem and progenitor cells and counteracts the erythroid-specific transcription factor GATA-1, to modulate the proliferation and differentiation of hematopoietic cells. During hematopoietic cell differentiation, GATA-2 exhibits dynamic expression patterns, which are regulated by multiple transcription factors....

Hallgren, J., 2004. The role of heparin in the activation of mast cell tryptase. Doctor’s dissertation. ISSN 1401-6257, ISBN 91-576-6676-8 Mast cells play an important role in our immune defense against bacteria and parasites but are also key effector cells in various inflammatory diseases. They act by releasing inflammatory mediators from intracellular granules. Tryptase, one of the most abund...

objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. one of the effective therapeutic methods for this disease can be gene therapy by viral vectors. the capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini lcr and β-globin gene instead of lcr region. the aim of this study is to make a recombinant lentiviruses conta...

Ataxia-telangiectasia (A-T) is a multisystem hereditary disease fea turing neurodegeneration, immunodeficiency, extreme cancer proneness, chromosomal instability, and radiosensitivity. A-T is found in many ethnic groups, and is genetically heterogeneous: four complementation groups have been identified in A-T so far. Attempts to isolate the A-T gene are based in part on gene transfer experiment...

S OF SPECIAL INTEREST NIOLECULAR AGING OF RABBIT HEMOGLOBIN DURING RED CELL LIFE SPAN. J. Rosa, G. Schapira and J. C. Dreyfus. From the H#{244}pital des EnfantsNlalades, Paris. Bull. Soc. chim. biol., 43: (4) 1961. On starch block electrophoresis, rabbit hemoglobin fractionates into a main fraction L1 and a minor fraction L5, incompletely separated. The use of valine-C14 and arginine-C14 as lab...

In this study, we investigated the incorporation of poly(vinyl alcohol) (PVA) nanofibers within poly(methyl methacrylate) (PMMA) microchannels in order to allow for sample preparation and concentration within microfluidic detection systems. Nanofibers were electrospun onto gold microelectrodes and integrated into polymer microfluidic systems using ultraviolet (UV)-assisted thermal bonding. We d...

S OF SPECIAL INTEREST NIOLECULAR AGING OF RABBIT HEMOGLOBIN DURING RED CELL LIFE SPAN. J. Rosa, G. Schapira and J. C. Dreyfus. From the H#{244}pital des EnfantsNlalades, Paris. Bull. Soc. chim. biol., 43: (4) 1961. On starch block electrophoresis, rabbit hemoglobin fractionates into a main fraction L1 and a minor fraction L5, incompletely separated. The use of valine-C14 and arginine-C14 as lab...