Complex carbohydrates on the surfaces of eukaryotic cells are thought to participate in a wide variety of cell-cell interactions. A model system has therefore been developed to study these processes. In the present experiments, the ability of chicken hepatocytes to recognize and adhere to sugars covalently linked to polyacrylamide gels was investigated. The gels were snythesized by two methods....

Experiments in the dynamic fracture of brittle polyacrylamide gels show that a single-crack state undergoes a hysteretic transition to the microbranching instability with a characteristic activation time. Quantitative measurements also indicate that features such as crack front inertia, self-focusing of microbranches, and the appearance of front waves are universal attributes of dynamic fracture.

1. The absolute electrophoretic mobilities of eight proteins have been measured at pH8.76, I 0.05, in polyacrylamide gels of 20 different compositions at 10 degrees C. 2. The partition coefficients of these proteins have been determined chromatographically under the same conditions by using columns of granulated polyacrylamide gel prepared simultaneously. 3. The electrophoretic mobilities are a...

The study takes up the problem whether synthesis of certain protein fractions in nerve cells of the hippocampus in rats during the transfer of handedness may be specific for this learning process. Electrophoretic separation of protein was carried out on polyacrylamide gels at the microscale. The investigation encompasses the brain-specific, acidic protein S100 and two protein fractions moving c...

D ISCOT1XUOUS ELECT1IO1’HORESIS in polyacrylamide rods has been used for separation of serum proteins (1). In spite of satisfactory resolution in tile rod, the technic renhains cumbersome, and comparison of the closely migrating cOnlpoflents inaccurate. Akroyd (2) reported discontinuous electrophoresis On polyacrylamide gel slab, which alleviates many of the difficulties of the rod. We llave co...

The synthesis and physicochemical properties of hydrogels with interpenetrated physical chemical networks were considered in relation to their prospective application as biomimetic materials biomedicine bioengineering. study was focused on combined based natural polysaccharide—calcium alginate (CaAlg) a synthetic polymer–polyacrylamide (PAAm). series varying proportions among polyacrylamide hav...

A new method for on-chip sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins is reported. Miniaturization of SDS-PAGE has attracted significant attention because it offers rapid analysis times, excellent resolution, high throughput, and the potential for integration and automation, as compared to conventional counterparts. The presented on-chip SDS-PAGE technique em...

Thin gels (<I mm) can be loaded conveniently, rapidly, and quantitatively by suspending the sample to be analyzed in a drop of agarose gel and simply placing the solidified drop on top of the stacking gel. With this method there is no lower limit to the size of the sample to be loaded or to the thinness of the gel to be employed. Using the silver stain quantitation of between 1 and 1000 @ample ...

Designed for compatibility with slab-gel polyacrylamide gel electrophoresis (PAGE) reagents and instruments, we detail development of free-standing polyacrylamide gel (fsPAG) microstructures supporting electrophoretic performance rivalling that of microfluidic platforms. For the protein electrophoresis study described here, fsPAGE lanes are comprised of a sample reservoir and contiguous separat...