The textile industry is one of the biggest industries in the world and its wastewater is one of the most important threats to the environment and human health. In this study, the ability of Haloarcula sp. IRU1 to produce poly(3-hydroxybutyrate) (PHB) was investigated using a basal salts medium. Different factors like textile wastewater, yeast extract, and KH2PO4 (as carbon, nitrogen, and phosph...

A novel, quantitative method for detecting poly-3-hydroxybutyrate (PHB) amounts in viable cells was developed to allow for high-throughput screening of mutant libraries. The staining technique was demonstrated and optimized for the cyanobacterium Synechocystis sp. strain PCC6803 and the eubacterium Escherichia coli to maximize the fluorescence difference between PHB-accumulating and control cel...

Two types of polyhydroxyalkanoate (PHA) biosynthesis gene loci (phb and pha) of Pseudomonas sp. strain 61-3, which produces a blend of poly(3-hydroxybutyrate) [P(3HB)] homopolymer and a random copolymer (poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) [P(3HB-co-3HA]) consisting of 3HA units of 4 to 12 carbon atoms, were cloned and analyzed at the molecular level. In the phb locus, three open read...

The present study was aimed to evaluate the suitability of agro-wastes and crude vegetable oils for cost-effective production poly-?-hydroxybutyrate (PHB), growth kinetics PHB in Alcaligenes faecalis RZS4 Pseudomonas sp. RZS1 with these carbon substrates biodegradation accumulated by cultures. accumulates higher amounts corn (79.90% dry cell mass) rice straw (66.22% medium respectively. kinetic...

The degradation patterns of bacterial poly-3-hydroxybutyrate (PHB) in chloroform solution under the action thionyl chloride presence zinc were studied. When 2.5.mol and 100 mmol introduced into 25 PHB, a decrease molecular weight polymer was observed. During reaction, relatively rapid noted first hour experiment; thus, values weight-average decreased from 840 kDa to 483, 167, 58.6, 16.7 after 1...

This paper reports the hydrolytic degradation and protein release studies for a series of newly synthesized thermogelling tri-component multi-block poly(ether ester urethane)s consisting of poly[(R)-3-hydroxybutyrate] (PHB), poly(propylene glycol) (PPG), and poly(ethylene glycol) (PEG). The poly(PEG/PPG/PHB urethane) copolymer hydrogels were hydrolytically degraded in phosphate buffer at pH 7.4...

Poly(3-hydroxybutyrate) (PHB) production and mobilization in Ralstonia eutropha are well studied, but in only a few instances has PHB production been explored in relation to other cellular processes. We examined the global gene expression of wild-type R. eutropha throughout the PHB cycle: growth on fructose, PHB production using fructose following ammonium depletion, and PHB utilization in the ...

Cocoa bean shell (CBS), a by-product of the chocolate industry, has been employed as substrate to obtain poly(3-hydroxybutyrate) (PHB) by fermentation with Bacillus firmus. With this aim, acid-thermal hydrolysis CBS (20% w/v) was conducted at 135 °C for 10 min so that broths rich in fermentable sugars were obtained. These broths, both non-centrifuged and centrifuged, media. Significant polymer ...

Differences in carbon assimilation pathways and reducing power requirements among organisms are likely to affect the role of the storage polymer poly-3-hydroxybutyrate (PHB). Previous researchers have demonstrated that PHB functions as a sole growth substrate in aerobic cultures enriched on acetate during periods of carbon deficiency, but it is uncertain how C(1) metabolism affects the role of ...

Based on direct sucrose conversion, the bacterium Burkholderia sacchari is an excellent producer of the microbial homopolyester poly(3-hydroxybutyrate) (PHB). Restrictions of the strain's wild type in metabolizing structurally related 3-hydroxyvalerate (3HV) precursors towards 3HV-containing polyhydroxyalkanoate (PHA) copolyester calls for alternatives. We demonstrate the highly productive bios...