DNA synthesis in Escherichia coli is inhibited transiently after UV irradiation. Induced replisome reactivation or "replication restart" occurs shortly thereafter, allowing cells to complete replication of damaged genomes. At the present time, the molecular mechanism underlying replication restart is not understood. DNA polymerase II (pol II), encoded by the dinA (polB) gene, is induced as part...

In most double-stranded RNA (dsRNA) viruses, RNA transcription occurs inside a polymerase (Pol) complex particle, which contains an RNA-dependent RNA Pol subunit as a minor component. Only plus- but not minus-sense copies of genomic segments are produced during this reaction. In the case of phi6, a dsRNA bacteriophage from the Cystoviridae family, isolated Pol synthesizes predominantly plus str...

Reactive oxygen species induce oxidative damage in DNA precursors, i.e. dNTPs, leading to point mutations upon incorporation. Escherichia coli mutT strains, deficient in the activity hydrolysing 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP), display more than a 100-fold higher spontaneous mutation frequency over the wild-type strain. 8-oxo-dGTP induces A to C transversions wh...

We investigate the polynomial closure operation (C 7→ Pol (C)) defined on classes of regular languages. We present an interesting and useful connection relating the separation problem for the class C and the membership problem for it polynomial closure Pol (C). It was first discovered in [6]. This connection is formulated as an algebraic characterization of Pol (C) which holds when C is an arbi...

Transcription factor IIS (TFIIS) is a protein known for catalyzing the cleavage reaction of the 3'-end of backtracked RNA transcript, allowing RNA polymerase II (Pol II) to reactivate the transcription process from the arrested state. Recent structural studies have provided a molecular basis of protein-protein interaction between TFIIS and Pol II. However, the detailed dynamic conformational ch...

Diarrhea is one of the most common causes death in young piglets. Porcine rotavirus (PoRV) belongs to genus Rotavirus within family Reoviridae, and considered be primary pathogen causing diarrhea Portulaca oleracea L. (POL) has been reported alleviate viral infections. However, antiviral effect polysaccharide (POL-P), an active component POL, on PoRV infection remains unclear. This study demons...

Polarized Galactic synchrotron emission is an undesirable foreground for cosmic microwave background (CMB) experiments observing at frequencies $< 150$ GHz. We perform a combined analysis of observational data 1.4, 2.3, 23, 30 and 33 GHz to quantify the spatial variation polarized spectral index, $\beta^{pol}$, on $\sim3.5^\circ$ scales. compare results from different combinations address limit...

Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. For two well-defined Pol II-transcribed genes, CYC1 and PMA1, we demonstrate that both Rat1p exonuclease and Sen1p helicase are required for efficient termination by promoting degradation of the nascent transcript associated with Pol ...

Previous studies have shown that the small subunit of Xenopus DNA polymerase gamma (pol gammaB) acts as a processivity factor to stimulate the 140 kDa catalytic subunit of human DNA polymerase gamma. A putative human pol gammaB initially identified by analysis of DNA sequence had not been shown to be functional, and appeared to be an incomplete clone. In this paper, we report the cloning of ful...

UNLABELLED Escherichia coli's DNA polymerase IV (Pol IV/DinB), a member of the Y family of error-prone polymerases, is induced during the SOS response to DNA damage and is responsible for translesion bypass and adaptive (stress-induced) mutation. In this study, the localization of Pol IV after DNA damage was followed using fluorescent fusions. After exposure of E. coli to DNA-damaging agents, f...