Background: The refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. Objectives: The purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produ...

This paper is to estimate the optimum plot size with the shape for field research experiments on paddy yield trial considering the effect of plot size on variability in yield of crop as well as studying the coefficients of variation of different plot sizes and shapes of plots. The maximum curvature technique and comparable variance methods were exercised to estimate optimum plot size and shape ...

Numerical data for the distribution of citations are examined for: (i) papers published in 1981 in journals which are catalogued by the Institute for Scientific Information (783,339 papers) and (ii) 20 years of publications in Physical Review D, vols. 11-50 (24,296 papers). A Zipf plot of the number of citations to a given paper versus its citation rank appears to be consistent with a power-law...

Using dual-wavelength spectrometry, we have refined the mordant-based bilirubin slide method (Clin Chem 28:2366-2372, 1982) to co-detect unconjugated bilirubin (Bu) and its sugar conjugates (Bc) in 10 microL of serum. The assay is based on three principles: (a) mono- and diconjugated bilirubins behave spectrally like one fraction (Bc) when bound to the mordant, (b) Bu and Bc are spectrally dist...

We address optimal placement of vehicles with simple motion, to intercept a mobile target that arrives stochastically on a line segment. The optimality of vehicle placement is measured through a cost function associated with intercepting the target. With a single vehicle, we assume that the target either moves with fixed speed and in a fixed direction or moves to maximize the vertical height or...

Logistic regression with 1 regularization has been proposed as a promising method for feature selection in classification problems. Several specialized solution methods have been proposed for 1-regularized logistic regression problems (LRPs). However, existing methods do not scale well to large problems that arise in many practical settings. In this paper we describe an efficient interior-point...

We investigated effects of nutrient availability on shoot structure and light-interception efficiency based on data from control (C) and irrigated + fertilized (IL) trees of Norway spruce (Picea abies (L.) Karst.). The sampling of 1-year-old shoots was designed to cover the variation in canopy exposure within the live crown zone, where current-year shoots were still found. Canopy openness was u...