Sexual reproduction in eukaryotes implies a biphasic life cycle with alternating haploid and diploid phases. The nature of the biphasic life cycle varies markedly across taxa, and often either the diploid or the haploid phase is predominant. Why some taxa spend a major part of their life cycle as diploids and others as haploids remains a conundrum. Furthermore, ploidy levels may not only vary a...

We investigated the effects of various concentrations of diethyl aminoethyl hexanoate (DA-6) on the regeneration and growth of adventitious buds in in vitro purple coneflower cultures. Among the 3 types of explants tested, leaf explants required higher concentrations of DA-6 than petiole and root explants in order to obtain high regeneration rates, while root explants required the lowest concen...

Ploidy variation is found in contexts as diverse as solid tumors, drug resistance in fungal infection, and normal development. Altering chromosome or genome copy number supports adaptation to fluctuating environments but is also associated with fitness defects attributed to protein imbalances. Both aneuploidy and polyploidy can arise from multinucleate states after failed cytokinesis or cell fu...

Saccharum spontaneum L. is a crucial wild parent of modern sugarcane cultivars whose ploidy clones have been utilized successfully in improving the stress resistance and yield related traits of sugarcane cultivars. To establish knowledge regarding the genetic variances and evolutional relationships of ploidy clones of Saccharum spontaneum collected in China, the rDNA-ITS sequences of 62 ploidy ...

OBJECTIVES To assess the prognostic value of flow cytometry and nuclear morphometry in prostate cancer after androgen deprivation treatment. METHODS A total of 127 patients with a prostate cancer diagnosis who had undergone androgen suppression were retrospectively studied. The DNA content by flow cytometry and nuclear morphometry was studied from biopsy specimens. In the patients with Stage ...

Plant size is largely determined by the size of individual cells. A number of studies showed a link between ploidy and cell size in land plants, but this link remains controversial. In this study, post-germination growth, which occurs entirely by cell elongation, was examined in diploid and autotetraploid hypocotyls of Arabidopsis thaliana (L.) Heynh. Final hypocotyl length was longer in tetrap...

DNA ploidy and chromosome (FISH) pattern analysis of peripheral nerve sheath tumors To the Editor, This report contains highly informative image DNA cytometry and Fluorescence in situ Hybridization (FISH) data on the DNA ploidy analysis of peripheral nerve sheath tumors [3]. The FISH analysis used DNA probes specific for centromeric regions combined with automatic image analysis. The data suppo...

Plant biotrophs often establish highly specialized and localized interaction sites where sustained nutrient exchange occurs. Increased plant nuclear DNA ploidy at or adjacent to these sites has now been reported for a diverse set of interactions, including those with fungal and bacterial symbionts and parasitic fungi and nematodes. Also, novel regulators of induced endoreduplication have recent...

The presence of high-ploidy cells in malignant tumours has long been documented. However, the biological significance of these cells is not known and there is a great deal of controversy over their proliferative potential. We have analysed the behaviour of these cells in two murine tumour lines, B16F10 melanoma and 3T3A31M angiosarcoma, determining their DNA content by microspectrophotometry an...