Advances in fluorescence in situ hybridization (FISH) make it feasible to detect multiple copy-number changes in hundreds of cells of solid tumors. Studies using FISH, sequencing, and other technologies have revealed substantial intra-tumor heterogeneity. The evolution of subclones in tumors may be modeled by phylogenies. Tumors often harbor aneuploid or polyploid cell populations. Using a FISH...

flow cytometry (fcm) was used to determine the ploidy levels of six different populationsof agropyron cristatum (l.) gaertn. (poaceae) in iran, using a diploid cultivated barley (hordeumvulgare l.) as an internal reference. according to flow cytometric data, tetraploid (2n = ± 28) andhexaploid (2n = ± 42) levels were detected among the examined populations and these results weresupported by chr...

Detection of relapse in acute lymphoblastic leukemia (ALL) is essential for proper management. However, immunophenotypic detection of relapse by flow cytometry in B-lineage ALL can be confounded by several factors, including lack of a unique immunophenotype and modulation of aberrant phenotypes after treatment. We hypothesized that flow cytometric DNA ploidy analysis may detect relapse in aneup...

Purpose. The purpose of this study was to evaluate: (1) the correlation between grade and ploidy or S-phase fraction (SPF), (2) the prognostic value of DNA flow cytometric study in soft tissue sarcomas.Patients /Methods. In all, 47 tissue samples from soft tissue sarcoma patients, surgically treated in the same center, were included. Flow cytometric analyses were performed according to a modifi...

BACKGROUND The diagnosis of uterine sarcoma is associated with poor outcome for the patient and there is a need for reliable prognostic markers. Most previous studies on the prognostic value of DNA ploidy include few uterine sarcomas and report conflicting results. MATERIALS AND METHODS We examined the prognostic value of DNA ploidy and its association with clinicopathological parameters and ...

Flow cytometry (FCM) was adapted to measure ploidy values in megakaryocytes isolated from normal guinea pigs and African green monkeys. The FCM approach was validated by microscopic identification of electronically sorted SN and higher ploidy cells and by comparisons with Feulgen microdensitometry on the same samples. Sorted 8N populations contained only megakaryocytes and were completely free ...

Sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. However, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. The aim of this study was to compare the response of triploid Atlantic salmon to a ...

Honey bees (Apis mellifera) exhibit age polyethism, whereby female workers assume increasingly complex colony tasks as they age. While changes in DNA methylation accompany age polyethism, other DNA modifications accompanying age polyethism are less known. Changes in endopolyploidy (DNA amplification in the absence of cell division) with increased larval age are typical in many insect cells and ...

Abstract Resource limitations shape the outcome of competitions between heterogeneous pre-malignant cells. One example such heterogeneity is in ploidy (DNA content) High-ploidy cells need more resources, to synthesize increasing amounts DNA, RNA and proteins. To model how subpopulations with variable DNA-content compete resource limited environment human brain we developed a stochastic state-sp...