Platelets from a patient with a mild inherited bleeding disorder and abnormal platelet aggregation and secretion show reduced generation of inositol 1.4.5-trisphosphate, mobilization of intracellular Ca2+, and phosphorylation of pleckstrin in response to several G protein mediated agonists, suggesting a possible defect at the level of phospholipase C (PLC) activation (see accompanying report). ...

The Gray Platelet Syndrome (GPS) is a rare inherited bleeding disorder characterized by deficiency of platelet α-granules, macrothrombocytopenia and marrow fibrosis. The autosomal recessive form of GPS is linked to loss of function mutations in NBEAL2, which is predicted to regulate granule trafficking in megakaryocytes, the platelet progenitors. We report the first analysis of cultured megakar...

Von Willebrand disease (VWD) is the common autosomal bleeding disorder caused by a quantitative and/ or qualitative defect in the Von Willebrand Factor (VWF), a large multimeric multifunctional plasma glycoprotein which plays a critical role in hemostasis. It is essential for platelet adhesion to damaged endothelium as well as platelet interactions at high shear stress. VWF has a direct role in...

The template bleeding time is a measure of platelet participation in primary hemostasis. Aspirin alters platelet function through interference with prostaglandin biosynthesis. In many individuals, aspirin will consistently prolong the bleeding time. Despite this observation, normal individuals rarely develop a bleeding disorder. This prompted us to investigate the influence of technical variabl...

Von Willebrand disease (VWD) is the common autosomal bleeding disorder caused by a quantitative and/ or qualitative defect in the Von Willebrand Factor (VWF), a large multimeric multifunctional plasma glycoprotein which plays a critical role in hemostasis. It is essential for platelet adhesion to damaged endothelium as well as platelet interactions at high shear stress. VWF has a direct role in...

Immune thrombocytopenic purpura (ITP) is an autoimmune disorder that is characterized by antibody-mediated platelet destruction and decreased platelet production. ITP and its treatments have been recognized to cause diminished quality of life in those afflicted with this illness on levels comparable to other chronic diseases. The disease can be self-limiting, but in adults it often is a chronic...

The template bleeding time is a measure of platelet participation in primary hemostasis. Aspirin alters platelet function through interference with prostaglandin biosynthesis. In many individuals, aspirin will consistently prolong the bleeding time. Despite this observation, normal individuals rarely develop a bleeding disorder. This prompted us to investigate the influence of technical variabl...

The template bleeding time is a measure of platelet participation in primary hemostasis. Aspirin alters platelet function through interference with prostaglandin biosynthesis. In many individuals, aspirin will consistently prolong the bleeding time. Despite this observation, normal individuals rarely develop a bleeding disorder. This prompted us to investigate the influence of technical variabl...

The template bleeding time is a measure of platelet participation in primary hemostasis. Aspirin alters platelet function through interference with prostaglandin biosynthesis. In many individuals, aspirin will consistently prolong the bleeding time. Despite this observation, normal individuals rarely develop a bleeding disorder. This prompted us to investigate the influence of technical variabl...

End-stage renal disease patients, particularly those treated with hemodialysis (HD), suffer from complex hemostatic disorders. Patients with uremia may experience two opposite he‐ mostatic complications: bleeding diathesis and thrombotic tendencies. Bleeding diathesis in uremic patients is primarily seen due to abnormalities in primary hemostasis, particularly platelet function disorder and imp...