RecA protein catalyzes DNA strand exchange, a basic step of homologous recombination. Upon binding to single-stranded DNA (ssDNA), RecA protein forms a helical nucleoprotein filament. Normally, this nucleoprotein filament binds double-stranded DNA (dsDNA) and promotes exchange of base pairs between this dsDNA and the homologous ssDNA that is contained within this filament. Here, we demonstrate ...

Remodeling of actin filament arrays in response to biotic and abiotic stimuli is thought to require precise control over the generation and availability of filament ends. Heterodimeric capping protein (CP) is an abundant filament capper, and its activity is inhibited by membrane signaling phospholipids in vitro. How exactly CP modulates the properties of filament ends in cells and whether its a...

BACKGROUND Mild hypertrophy but increased arrhythmic risk characterizes the stereotypic phenotype proposed for hypertrophic cardiomyopathy (HCM) caused by thin-filament mutations. However, whether such clinical profile is different from more prevalent thick-filament-associated disease is unresolved. OBJECTIVES This study aimed to assess clinical features and outcomes in a large cohort of pati...

This paper reports the computer simulation of a flapping flexible filament in a flowing soap film using the immersed boundary method. Our mathematical formulation includes filament mass and elasticity, gravity, air resistance, and the two wires that bound the flowing soap film. The incompressible viscous Navier–Stokes equations, which are used to describe the motion of the soap film and filamen...

Additive manufactured parts often require post-processing to obtain the desired surface finish. In this sense, work investigates ball burnishing process’s applicability as a finishing method for fused filament fabricated (FFF) parts. Ball is mechanical procedure based on plastic deformation of treated that could provide significant advantages, it non-abrasive and does not chemically interact wi...

Observations are presented of several novel phenomena involved in the dynamics of a pendant drop of viscoelastic micellar fluid falling through air. Generally, when a drop falls a filament forms connecting it to the orifice; the filament eventually breaks due to an instability. The filament dynamics and instabilities reported here are very different from the standard Newtonian and non-Newtonian...

On 2005 September 13 a filament eruption accompanied by a halo CME occurred in the most flare-productive active region NOAA 10808 in Solar Cycle 23. Using multi-wavelength observations before the filament eruption on Sep. 13th, we investigate the processes leading to the catastrophic eruption. We find that the filament slowly ascended at a speed of 0.1km s over two days before the eruption. Dur...

Capping proteins limit actin filament growth, but paradoxically increase actin-based cell motility. This has been attributed to funneling of actin monomers to the filament ends that remain uncapped. Using a reconstituted motility system, Akin and Mullins (2008) now demonstrate that filament capping increases Arp2/3-based nucleation and branching, rather than elevating the rate of filament elong...

Myosin V is a two-headed processive motor protein that walks in a hand-over-hand fashion along actin filaments. When it encounters a filament branch, formed by the Arp2/3 complex, it can either stay on the straight mother filament, or switch to the daughter filament. We study both probabilities using the elastic lever arm model for myosin V. We calculate the shapes and bending energies of all r...