J. B. Egan and M. L. Morse (Biochim. Biophys. Acta 97:310, 1965) reported a defined medium which would support the growth of Staphylococcus aureus NCTC8511 for several generations if a peptone medium culture was diluted into it. In our hands, cultures of S. aureus NCTC8511 or S. aureus HS1159 (obtained from R. Z. Korman, Department of Physical Biology, Cornell University, Ithaca, N.Y.) maintain...