Inhalation of fluorocarbon polymers can cause pulmonary toxicity. Although multiple cases of lung injury have been reported, cellular characterization of the associated alveolitis occurring acutely after inhalation is limited. We report the case of a previously healthy woman who presented at our Emergency Department with an acute pneumonitis following inhalation of a fluorocarbon polymer-based ...

objective the aim of this study was to compare the sensitivity and specificity of acid fast and auramine-rhodamine staining and multiplex pcr for the detection of mycobacterium tuberculosiscomplex and non tuberculosis mycobacteria on formalin fixed paraffin embedded tissues (ffpe) materials and methods forty cases of ffpe pleural and bronchial tissue with chronic granulomatous inflammation and ...

We present a large comparative series of trypan blue use in cataract surgery. This series of trypan blue used in all eyes regardless of cataract severity may be unique. We found an apparent increased rate of cystoid macular oedema (CMO) associated with trypan blue use. Melles et al’s report on the use of trypan blue in cataract extraction in 1999 combined with Apple et al’s series on dye enhanc...

The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by SYTO 13; conversely, gram-positive organisms were rendere...

Benign cephalic histiocytosis (BCH) is a rare type of non-Langerhans histiocytosis with features of many small yellow, yellow-red, or yellow-brown papules located on face, head, neck, and shoulders of infants and young children. There would be no associated symptoms. The histopathological images reveal infiltration of histiocytes in dermis; immunohistochemically, the histiocytes are positive fo...

Crude ethanolic extract and column chromatographic fractions of the Allepey cultivar of Curcuma longa Roxb, commonly called turmeric (tumeric) in commerce, were used as a stain for tissue sections. Staining was carried out under basic, acidic and neutral media conditions. Inorganic and organic dissolution solvents were used. The stain was used as a counterstain after alum and iron haematoxylins...

Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed...

A direct staining technique was investigated for the detection of viable Salmonella in fresh and processed meats. The technique involved overnight enrichment in BPW, extraction of Salmonella cells onto a polycarbonate membrane, followed by detection of the pathogen using anti-Salmonella monoclonal antibody coupled with an antibody linked-fluorescent stain (Texas Red) and a viability stain (Syto...

Two carbonate stones (a limestone and a marble), commonly used for flooring, wall cladding countertops, were exposed to different staining agents. After each cycle (staining cleaning with non-ionic detergent solution), the surface degradation was assessed on scale ranging from unchanged severe change (based qualitative characterization presented standard UNE-EN 16301:2014), stereomicroscopy col...