BACKGROUND Non-conventional yeasts present a huge, yet barely exploited, resource of yeast biodiversity for industrial applications. This presents a great opportunity to explore alternative ethanol-fermenting yeasts that are more adapted to some of the stress factors present in the harsh environmental conditions in second-generation (2G) bioethanol fermentation. Extremely tolerant yeast species...

The present work is initiated to investigate whether a defined culture comprising a mixture of three yeast species, Kluyveromyces marxianus, Saccharomyces cerevisiae, and Pichia stipitis can ferment a mixture of sugars to produce bioethanol at rates higher than those achieved by pure cultures of the same. For this purpose, we develop models of single species based on the hybrid cybernetic model...

to accomplish the worldwide demand for recombinant human erythropoietin (rhuepo) as a therapeutic, application of cost-efficient expression system of methylotrophic yeast pichia pastoris (p. pastoris) rather than mammalian cells is indispensable. herein, a report on high levels secreted-expression of pichia-derived rhuepo by batch fermentation in a ph stabilized format is presented. the full le...

phytic acid is the dominant form of phosphorous in plant seeds. however, phytic acid cannot beutilized by animals and causes them serious phosphate deficiency. utilization of phytase as ananimal feed additive can affect liberation of phosphor and its mineral availability. in this study,heterologous expression of the a. niger phya gene was investigated in the methylotrophic yeastsp. pastoris and...

Resident fruit microflora has been the source of biocontrol agents for the control of postharvest decay of fruits and the active ingredient in commercialized biocontrol products. With the exception of grapes and apples, information on the resident microflora of other fruits is only fragmentary, but greater knowledge in this area can be very helpful in developing biocontrol strategies. We charac...

The phytase gene appAS was isolated from Shigella sp. CD2 genomic library. The 3.8 kb DNA fragment contained 1299 bp open reading frame encoding 432 amino acid protein (AppAS) with 22 amino acid signal peptide at N-terminal and three sites of N-glycosylation. AppAS contained the active site RHGXRXP and HDTN sequence motifs, which are conserved among histidine acid phosphatases. It showed maximu...

A recombinant C-terminus heavy chain fragment from botulinum neurotoxin serotype E (BoNT/E) is proposed as a vaccine against the serotype E neurotoxin. This fragment, rBoNTE(Hc), was produced intracellular in Pichia pastoris GS115 by a three-step fermentation process, i.e., glycerol batch phase and a glycerol fed-batch phase to achieve high cell densities, followed by a methanol fed-batch induc...

An unspecific peroxygenase from Aspergillus brasiliensis highly expressed in Pichia pastoris catalyses aromatic hydroxylation of a number benzylic compounds.