Pokeweed antiviral proteins enzymatically inactivate the 60 S subunit of eucaryotic ribosomes in cell-free preparations. Three different species of the enzyme can be isolated from spring leaves, summer leaves, and seeds of pokeweed. Sequence analyses of the NH2-terminal residues show that pokeweed antiviral protein, isolated from spring leaves and seeds, are homologous and differ in 11 of the 2...

Some environmental issues polarize people, producing weary political stalemates of indecision and inaction. Others, however, grab hold of our most primeval instincts, causing us to reach deeply into our memories of childhood, and our first direct experiences with nature: the bumble bee nest we poked at with a stick; the man at the county fair with the bee beard. Those memories expand backward i...

The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in thymidine incorporation and cell number. The concentration of LPS maximally stimulating fibroblasts...

Blast transformation of lymphocytes from diabetics and nondiabetics was evaluated after adding insulin at various concentrations. Responses to phytohemagglutinin-P (PHA-P), concanavalin-A (CON-A) and pokeweed mitogen (PWM) were measured in the presence of exogenous insulin added in physiologic increments of 0, 10, 20, 30, and 40 microunits of activity per ml of culture medium. A modified method...

Facebook is one of the most popular Internet sites today. A key feature that arguably contributed to Facebook’s unprecedented success is its application platform, which enables the development of third-party social-networking applications. Understanding how these applications are installed and used is important for the function and utility of web-based online social networks, e.g. to better eng...

BACKGROUND Sarma - cooked leaves rolled around a filling made from rice and/or minced meat, possibly vegetables and seasoning plants - represents one of the most widespread feasting dishes of the Middle Eastern and South-Eastern European cuisines. Although cabbage and grape vine sarma is well-known worldwide, the use of alternative plant leaves remains largely unexplored. The aim of this resear...

Olfaction based behavioral experiments are important for the investigation of sensory coding, perception, decision making and memory formation. The predominant experimental paradigms employ forced choice operant assays, which require associative learning and reinforced training. Animal performance in these assays not only reflects odor perception but also the confidence in decision making and m...

A recently developed model posits that a bout of operant responding comprises three different components: bout initiation rate, within-bout response rate and bout length. Each parameter is thought to be affected by different classes of variables. Pentobarbital was used to assess the independence and sensitivity of these parameters in two mouse strains, BALB/c and C57BL/6, selected because of th...

A recent study by Dr. Maria Antonietta De Luca demonstrated intravenous (IV) self-administration responding (nose-poking) for the synthetic cannabinoid JWH-018 [1-pentyl-3-(1-naphthoyl)indole] (Figure 1) in an experimentally naïve, adult rat species [1]. This finding is unexpected since the phytocannabinoid (−)-trans-Δ9tetrahydrocannabinol (Δ9-THC, Figure 1), a primary psychoactive constituent ...

BACKGROUND Since the first texts on local anesthesia were written in the early 1900s, it has been widely quoted and believed that dorsal finger skin is less sensitive to needlestick pain than volar finger skin. The result is that the most commonly used finger block for local anesthesia is the dorsal two injection technique. METHODS In this study, the needlestick discomfort associated with dor...