Bacillus species producing a thermostable phytase was isolated from soil, boiled rice, and mezu (Korean traditinal koji). The activity of phytase increased markedly at the late stationary phase. An extracellular phytase from Bacillus sp. KHU-10 was purified to homogeneity by acetone precipitation and DEAE-Sepharose and phenyl-Sepharose column chromatographies. Its molecular weight was estimated...

The aim of the study was to observe the effects of dietary available phosphorus (aP) and calcium (Ca), with regular or super doses of phytase, on phytate hydrolysis and subsequent influences on broiler growth performance and nutrient utilization. In a 2 × 3 factorial design, 384 Ross-308 broilers were allocated to one of 6 dietary treatments with 8 replicates in a randomized complete block desi...

A trial was conducted to evaluate pure NSP enzyme combination derived from in vitro studies and commercially available phytase to corn-soybean meal based low energy diets singly and combination of both. The experiment was conducted by using completely randomized design on one hundred and fifty layer birds (40 weeks) of uniform body weight and production with five treatments, six replicates and ...

Rice bran (RB) is a major by-product, produced from the rice milling process. This study was conducted to evaluate the performance, egg quality, and blood biochemical parameters of 288 Hy-line laying hens fed diets with rice bran and commercial microbial phytase Natuphos®5000. The experiment was a 4×2 factorial one with four RB levels (0, 5, 10, and 15%) by two phytase levels (0 and 5000 FTU/kg...

Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase fr...

Abstract Due to the significant role of bread in food basket people world, it is necessary consider its quality and nutritional attributes. significance alpha-amylase phytase enzymes sensitivity these some environmental conditions, was attempted optimize model encapsulation conditions combination two beeswax. The parameters amylase phytase, beeswax amount, tween 20, mixing speed were regarded a...

The inefficient digestion of phytate phosphorus by fish has created environmental concerns associated with phosphorus pollution from aquaculture production facilities. To further complicate this situation, phytate is known to chelate minerals and proteins, making them nutritionally unavailable. The enzyme phytase degrades phytate into inorganic phosphorus, which can be directly utilized by fish...

A neutral phytase from germinating mung bean (Vigna radiata) seeds dephosphorylates myo-inositol hexakisphosphate sequentially to myo-inositol. The enzyme also binds with higher affinity to myo-inositol trisphosphates (1,4,5), (2,4,5), and (1,3,4) isomers without catalysis. The high affinity complex elicits Ca(2+) mobilization in vitro from microsomes/vacuoles via the formation of a ternary com...

The effects of phytic acid and 2 sources of exogenous phytase (bacterial vs. fungal) on the flow of endogenous amino acids at the terminal ileum of broilers were assessed using the enzyme-hydrolyzed casein method. Phytic acid (as the sodium salt) was included in a purified diet at 8.5 and 14.5 g/kg, and each diet was fed without or with a fungal (Aspergillus niger-derived) or a bacterial (Esche...

During the past decade, the inclusion of microbial phytase in poultry diets has increased remarkably, mainly in response to heightened concerns over phosphorus (P) pollution of the environment. The capacity of this feed enzyme to release phytate-bound P and reduce P excretion is now well documented. Effectively, phytase is an alternative, economical P source and, as global phosphate reserves ar...