PEP mutase catalyzes the conversion of phosphoenolpyruvate (PEP) to phosphonopyruvate in biosynthetic pathways leading to phosphonate secondary metabolites. A recent X-ray structure [Huang, K., Li, Z., Jia, Y., Dunaway-Mariano, D., and Herzberg, O. (1999) Structure (in press)] of the Mytilus edulis enzyme complexed with the Mg(II) cofactor and oxalate inhibitor reveals an alpha/beta-barrel back...

Enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system catalyzes phosphoryl transfer from phosphoenolpyruvate to the heat-stable phosphoryl carrier protein, HPr. Enzyme I also catalyzes a rapid phosphoryl exchange reaction in which the phosphoryl moiety of phosphoenolpyruvate is transferred to pyruvate. Additionally, Enzyme I plus HPr catalyze the slow hydrolysis of pho...

Selenophosphate synthetase, the Escherichia coli selD gene product, is a 37-kDa protein that catalyzes the synthesis of selenophosphate from ATP and selenide. In the absence of selenide, ATP is converted quantitatively to AMP and two orthophosphates in a very slow partial reaction. A monophosphorylated enzyme derivative containing the gamma-phosphoryl group of ATP has been implicated as an inte...

Enzyme I (EI) is the first component in the bacterial phosphotransferase system, a signal transduction pathway in which phosphoryl transfer through a series of bimolecular protein-protein interactions is coupled to sugar transport across the membrane. EI is a multidomain, 128-kDa homodimer that has been shown to exist in two conformational states related to one another by two large (50-90°) rig...

Phosphoryl transferase, a mitochondrial protein which increases the phosphorylative capacity of poorly phosphorylating submitochondrial particles and catalyzes an ATP-ADP exchange reaction, is phosphorylated during oxidation either of succinate or pyruvate-malate. Inhibitors of oxidative phosphorylation and electron transfer, as well as uncouplers of oxidative phosphorylation, inhibit the phosp...

Enantioenriched secondary alcohols represent an important class of molecules found in numerous intermediates, chiral building blocks, and biologically active compounds. Asymmetric reduction of prochiral ketones constitutes the most straightforward way to form these important moieties. Traditionally, stoichiometric amounts of chiral ligands were used together with an aluminium or a boron hydride...

Organophosphates are organic substances that contain a phosphoryl or a thiophosphoryl bond. They are mainly used around the world as pesticides, but can also be used as chemical warfare agents. Their detection is normally entrusted to techniques like GC- and LC-MS that, although sensitive, do not allow their identification on site and in real time. We have approached their identification by exp...

This paper is devoted to computer-aided design of new extractants of the uranyl cation involving three main steps: (i) a QSPR study, (ii) generation and screening of a virtual combinatorial library, and (iii) synthesis of several predicted compounds and their experimental extraction studies. First, we performed a QSPR modeling of the distribution coefficient (logD) of uranyl extracted by phosph...