Changes in the environment from drug product to human physiology might lead physical and/or chemical modifications of protein drug, such as vivo aggregation and fragmentation. Although subcutaneous (SC) injection is a common route administration for therapeutic proteins, knowledge on stability SC tissue limited. In this study, we developed physiologic vitro model simulating patients. We assesse...

1) Skin or mucosal biopsy specimens are usually obtained by 3-5 mm punch or surgical biopsy. 2) Biopsy specimens are snap frozen. If there is a delay between biopsy and snap freezing, the specimen should be kept in cold saline. 3) Frozen 4-6μ sections are cut on a cryostat and placed on a glass slide before being air-dried for 15 minutes. 4) After rinsing in phosphate buffered saline (PBS), pH ...

The adsorption and penetration of intracellular naked vaccinia virus (INV) and extracellular enveloped vaccinia virus (EEV) were examined. The adsorption kinetics of INV and EEV were similar, but INV adsorption was found to be more sensitive to the adsorption environment than EEV. The PFU-to-particle ratio for the two virus particles indicated that EEV was approximately two times as infectious ...

A phosphate-buffered saline and a chemically defined synthetic medium for in vitro maintenance of imaginal discs of Drosophila melanogaster were developed. The composition of the chemically defined medium was varied in order to optimize the incorporation of tritiated uridine into RNA and tritiated amino acids into acid-insoluble protein. The optimal ranges obtained were: pH, 6.75-7.35; osmolari...

objectives: this study investigated the possible synergistic effect of simultaneous treatment of bone morphogenic protein (bmp)-4 as a chemical stimulator and static magnetic field (smf) as a physical stimulator on viability percent and proliferation rate in rat bone marrow stem cells. methods: passage 5 cells were trypsinized, and a cell suspension prepared after which the cells were counted ...

The study highlights the corrosion behavior of untreated and treated tantalum with addition trimanganese tetraoxide (Mn3O4) nanoparticles in simulated inflammatory media. anodic layer was produced on pure by anodization electrolytes composed ammonium fluoride, ethylene glycol, water. Nanoparticles were deposited uniformly surface anodized electrophoretic deposition (EPD) method. results reveale...

Intradermal tests were compared among 4 groups of Behçet's disease, other uveitis caused by unknown agent, sarcoidosis and SLE. The size of indurations of PPD, SK-SD and Candida was the largest in Behçet, followed by other uveitis, sarcoidosis and SLE in a descending order. Pustule formation was frequently seen in Behçet and other uveitis, in contrast to low incidence in sarcoidosis and not at ...

Fig. 3. Inhibition of binding of ['4C]pentobarbital to rabbit antiserum by nonradioactive pentobarbital in buffered saline (0 0 *), plasma (0 O), or urine (X X). Incubation medium consisted of 0.10 ml of normal rabbit serum, 0.10 nml of rabbit antiserum (0.4 mg of protein), 0.01 ml of ['4C]pentobarbital (O.1 nmole), and 0.01 ml of either standard unlabeled pentobarbital (1 to 100 ng) or unknown...

Fig. 3. Inhibition of binding of ['4C]pentobarbital to rabbit antiserum by nonradioactive pentobarbital in buffered saline (0 0 *), plasma (0 O), or urine (X X). Incubation medium consisted of 0.10 ml of normal rabbit serum, 0.10 nml of rabbit antiserum (0.4 mg of protein), 0.01 ml of ['4C]pentobarbital (O.1 nmole), and 0.01 ml of either standard unlabeled pentobarbital (1 to 100 ng) or unknown...