Results from the present study have shown that the ionic species of buffers, pH values and reaction temperature can affect the enzyme unit activities and product specificity of Toruzyme (Novo Nordisk A/S Bagsvaerd, Denmark) CGTase (cyclodextrin glucanotransferase). Applying a similar reaction environment (acetate buffer, pH 6.0; temperature, 60 degrees C), the CGTase was found to be capable of ...

In phosphate buffer, the degradation of ATZ by ozone/(O3/H2O2) under various circumstance was explored and mechanism dynamics were probed. The findings revealed that when maintaining reaction temperature at 25 °C, H2O2 concentration O3 20 mol/L mol/L, respectively. Moreover, rate 5 influence O3/H2O2 92.59% in buffer pH7. analysis showed HO• underwent co-oxidized oxidation ratios close to 1:1 ac...

During the course of studies made with rat liver enzymes, it was found that the activity of aldolase was lower when assayed in the presence of phosphate buffer than with tris(hydroxymethyl)aminomethane buffer in the incubation system (1). A similar effect had been observed with crystalline aldolase from rabbit muscle and was attributed to an enhancement of the enzyme activity (2, 3). However, s...

this study examined if pre-germination altered the water content and water activity, contents of phytate, total phenolic, (±)-catechin, quercetin and total antioxidant capacity of almond  (prunus dulcis)kernel. raw almond kernels were submerged for 15 hours in water, 0.02 mol dm-3 phosphate buffer solution (ph 5.0) and 0.02 mol dm-3 phosphate buffer solution (ph 7.0) at 25 and 40ºc, respectivel...

The stability of centrifugally purified PR8 influenza virus at pH 7 in 0.001, 0.01, 0.1, and 1.0 M phosphate buffers, in veronal and borate buffers, and in adjusted solutions of saline and distilled water was investigated. The results demonstrate that the stability of this virus can vary considerably at pH 7 depending upon the nature and concentration of the salts present. Borate, veronal, and ...

OBJECTIVE the aim of this study was to test two buffer solutions in order to attain a reliable and reproducible analysis of inflammatory cytokines (IL-1β, IL-6, TNF-α, OPG, OPN and OC), in gingival crevicular fluid (GCF) by flow cytometry. MATERIAL AND METHODS GCF samples from healthy volunteers were collected with perio-paper strips and diluted either in phosphate buffered saline (PBS) or Tr...

When living cells of Nitella are exposed to a solution of sodium acetate and are then placed in a solution of brilliant cresyl blue made up with a borate buffer mixture at pH 7.85, a decrease in the rate of penetration of dye is found, without any change in the pH value of the sap. It is assumed that this inhibiting effect is caused by the action of sodium on the protoplasm. This effect is not ...