The interiors of cells are crowded, thus making it important to assess the effects of macromolecules on the folding of proteins. Using the self-organized polymer (SOP) model, which is a coarse-grained representation of polypeptide chains, we probe the mechanical stability of ubiquitin (Ub) monomers and trimers ((Ub)(3)) in the presence of monodisperse spherical crowding agents. Crowding increas...

Recessed-tip microelectrodes were used to measure internal pH (pHi) in the fungus Neurospora, and to examine the response of pHi to several kinds of stress: changes of extracellular pH (pHo), inhibition of the principal proton pump in the plasma membrane, and inhibition of respiration. Under control conditions, at pHo = 5.8, pHi in Neurospora is 7.19 +/- 0.04. Changes of pHo between 3.9 and 9.3...

BACKGROUND AND PURPOSE MRI studies in patients with atherosclerosis often reveal ill-defined hyperintensity in the pons on T2-weighted images. This pontine hyperintensity (PHI) does not fulfill the criteria of a brain infarct, and its clinical relevance is not established. We examined the frequency, as well as the radiological and clinical correlates, of PHI in poststroke patients. METHODS Th...

Recessed-tip microelectrodes were used to measure internal pH (pHi) in the fungus Neurospora, and to examine the response of pHi to several kinds of stress : changes of extracellular pH (pHo), inhibition of the principal proton pump in the plasma membrane, and inhibition of respiration . Under control conditions, at pHo = 5.8, pHi in Neurospora is 7 .19 t 0.04. Changes of pH. between 3.9 and 9....

To establish a method of pHi regulation and to understand the pH regulation mechanism in the cell, we investigated the pHi response of unfertilized or fertilized eggs of sea urchin, applying sea water containing both weak permeant acid, acetic acid and/or base, ammonia, to eggs. Pyranine was employed as a pH indicator to measure intracellular pH (pHi) by microfluorometry. The unfertilized/ferti...

We evaluated the dye 4',5'-dimethyl-5-(and -6-) carboxyfluorescein (Me2CF) for determining the intracellular pH(pHi) of isolated, perfused proximal tubules of the salamander. The intracellular absorbance spectrum, corrected for the intrinsic absorbance of the tubule, was obtained once per second. The dye was incorporated into tubule cells by exposing them to the membrane-permeable precursor 4',...

in this paper we introduce the concept of entropy operator for continuous systems of finite topological entropy. it is shown that it generates the kolmogorov entropy as a special case. if $phi$ is invertible then the entropy operator is bounded with the topological entropy of $phi$ as its norm.

The newly isolated peptide PHI provoked a dose-dependent stimulation of glycogenolysis and gluconeogenesis in isolated rat hepatocytes; at 1 microM-PHI, both processes were increased 1.6-fold as compared with basal values. These PHI-mediated effects were accompanied by the activation of glycogen phosphorylase and the inactivation of pyruvate kinase. PHI (1 microM) also caused a 2-fold increase ...

Black Student Movement [2] Minority Business Student Alliance [3] National Panhellenic Council (Black Greek Council) [4] Carolina Indian Circle [5] Carolina Hispanic Association [6] Asian Student Association [7] Black Sororities and Fraternities Alpha Kappa Alpha [8] Omega Psi Phi [9] Alpha Phi Alpha [10] Phi Beta Sigma [11] Delta Sigma Theta [12] Sigma Gamma Rho [13] Kappa Alpha Psi [14] Zeta ...

Black Student Movement [2] Minority Business Student Alliance [3] National Panhellenic Council (Black Greek Council) [4] Carolina Indian Circle [5] Carolina Hispanic Association [6] Asian Student Association [7] Black Sororities and Fraternities Alpha Kappa Alpha [8] Omega Psi Phi [9] Alpha Phi Alpha [10] Phi Beta Sigma [11] Delta Sigma Theta [12] Sigma Gamma Rho [13] Kappa Alpha Psi [14] Zeta ...