Glutamine synthetases from roots, nodules, and leaves of Phaseolus vulgaris L. have been purified to homogeneity and their polypeptide composition determined.The leaf enzyme is composed of six polypeptides. The cytosolic fraction contains two 43,000 dalton polypeptides and the chloroplastic enzyme is formed by four 45,000 dalton polypeptides. Root glutamine synthetase consists only of the same ...

Volatile compounds of uncooked dry bean {Phaseolus vulgaris L.) cultivars representing 3 market classes (black, dark red kidney and pinto) grown in 2005 were isolated with headspace solid phase microextraction (HS-SPME), and analyzed with gas chromatography mass spectrometry (GCMS). A total of 62 volatiles consisting of aromatic hydrocarbons, aldehydes, alkanes, alcohols and ketones represented...

Measurements of net photosynthesis show that in Phaseolus vulgaris L. the cultivar Michelite-62 exceeds the cultivar Red Kidney in net CO(2) uptake by 23 to 31%. Data on translocation of pulse label indicate that export of a pulse of photosynthetically assimilated (14)C from the source leaf of either M-62 or Red Kidney follows an exponential pattern and shows an initial rapid phase followed by ...

Phylogeographic methods provide the tools to accurately access the geographic origin and diversification of crop species. In the present commentary, I urge the common bean community to face those methods and a tree-thinking mentality with regards to the long standing debate of the origin of common bean. Such efforts will ultimately bring back interest into wild bean studies and reinforce the un...

The effect of Zn nutrition on leaf starch metabolism was studied in two navy bean (Phaseolus vulgaris L.) varieties, Sanilac and Saginaw. Sanilac is much more susceptible to Zn deficiency than is Saginaw. The variables examined in these two strains were starch content, the activity of soluble starch synthetase (ADP-glucose: starch alpha-4-glucosyltransferase, EC 2.4.1.b), and the size and numbe...

To lower seed yield loss from directly harvested common bean or dry bean, height of the lower pod-bearing nodes needs to be raised. The objective of this greenhouse study was to stimulate lower stem elongation by gibberellic acid (GA3) of dry bean cultivars. Seeds of cv. Matterhorn, erect indeterminate Type II, and cv. Poncho, prostate indeterminate Type III, were dipped in GA3 at 62.5 to 16,00...

Wild accessions of crops and landraces are valuable genetic resources for plant breeding and for conserving alleles and gene combinations in planta. The primary genepool of cultivated common beans includes wild accessions of Phaseolus vulgaris. These are of the same species as the domesticates and therefore are easily crossable with cultivated accessions. Molecular marker assessment of wild bea...

The cytokinin-active ribonucleosides present in tRNA from etiolated Phaseolus vulgaris L. seedlings have been isolated and identified as cis-ribosylzeatin, 2-methylthio-ribosylzeatin, and N(6)-(Delta(2)-isopentenyl)-adenosine. The structures of the compounds were established on the basis of their chromatographic properties and the mass spectra of their permethylated and perdeuteromethylated der...

The sorption rate and metabolic fate of nitrogen dioxide, a major air pollutant, have been determined for Phaseolus vulgaris (L.). Sorption was determined kinetically by chemiluminescent monitoring of (15)NO(2) removal from the test atmosphere and directly by mass spectrometric analysis of nitrogen derived from the plant tissue. Sorptive processes were first order with respect to (15)NO(2) conc...

Of nine plant growth regulators (indoleacetic acid, 1-naphthalene acetic acid, gibberellic acid, giberellin 4/7, 6-benzylaminopurine, 6-furfurylaminopurine, abscisic acid, and 1-aminocyclopropane carboxylic acid) tested, only 6-benzylaminopurine and abscisic acid affected (14)C-photosynthate unloading from excised seed coats of Phaseolus vulgaris L. Unloading, in the presence of KCl, was stimul...